An efficient and cost-effective method of generating postnatal (P2-5) mouse primary hippocampal neuronal cultures

Kaar A.; Morley S. J.; Rae M. G.

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An efficient and cost-effective method of generating postnatal (P2-5) mouse primary hippocampal neuronal cultures

机译：产生后产后（P2-5）小鼠原发性海马神经元培养物的高效且经济效益的方法

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Background: Primary culture of postnatal central neurons is a widely used methodology for applications such as the investigation of neuronal development, protein trafficking/distribution and cellular signalling. However, successful production and maintenance of such cultures, particularly from postnatal animals, can be challenging. In attempting to surmount these difficulties, several disparate culturing methodologies have been developed. Such methodologies are centred on the identification and optimisation of critical steps and, as such, the protocols and reagents utilised can differ quite markedly from protocol to protocol, often with the suggestion that the use of a (usually expensive) proprietary reagent(s), lengthy substrate preparation and/or cell isolation techniques is/are necessary for successful culture preparation.

机译：背景：后期中枢神经元的原发性培养物是一种广泛使用的应用方法，例如神经元发育，蛋白质贩运/分布和细胞信号传导的研究。然而，成功的生产和维护这些文化，特别是从产后动物，可能是挑战性的。在试图超越这些困难时，已经开发了几种不同的培养方法。这些方法以识别和优化为中心的关键步骤，因此所使用的方案和试剂可以从协议到协议的情况下差异，通常是使用（通常昂贵的）专有试剂的建议，成功培养制剂是必需的冗长的底物制备和/或细胞分离技术。

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《Journal of Neuroscience Methods》 |2017年第2017期|共9页
作者
Kaar A.; Morley S. J.; Rae M. G.;
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作者单位

Univ Coll Cork Dept Physiol Western Gateway Bldg Cork Ireland;

Univ Coll Cork Dept Physiol Western Gateway Bldg Cork Ireland;

Univ Coll Cork Dept Physiol Western Gateway Bldg Cork Ireland;

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原文格式 PDF
正文语种 eng
中图分类人体生理学;
关键词
Primary neuronal culture; Hippocampal neurons; Postnatal; Trituration; Mouse;

机译：原发性神经元培养物;海马神经元;产后;氚;小鼠;

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1. An efficient and cost-effective method of generating postnatal (P2-5) mouse primary hippocampal neuronal cultures [J] . Kaar A., Morley S. J., Rae M. G. Journal of Neuroscience Methods . 2017,第期

机译：产生后产后（P2-5）小鼠原发性海马神经元培养物的高效且经济效益的方法
2. Augmentation of Polysialic Acid by Valproic Acid in Early Postnatal Mouse Hippocampus and Primary Cultured Hippocampal Neurons [J] . Takamitsu Natori, Fumiaki Kodaira, Takae Hirasawa Journal of Bioscience and Bioengineering . 2008,第2期

机译：丙戊酸对产后早期小鼠海马和原代培养海马神经元的唾液酸增强作用
3. Synaptoneurosome micromethod for fractionation of mouse and human brain, and primary neuronal cultures [J] . ChangJ.W., ArnoldM.M., RozenbaumA., Journal of Neuroscience Methods . 2012,第2期

机译：突触神经小体微方法可用于分离小鼠和人脑以及原代神经元培养
4. Uric acid prevents traumatic cell death and neuronal dysfunction in organotypic hippocampal slice cultures [C] . Goletiani C., Morrison B. IEEE Annual Northeast Bioengineering Conference . 2010

机译：尿酸预防器官型海马切片培养中的创伤性细胞死亡和神经元功能障碍
5. Neuregulin 1 Nuclear Signaling Is Important for Postnatal Hippocampal Neurogenesis and Neuronal Development [D] . Jone, Alice. 2019

机译：Neuregulin 1核信号传导对于产后海马神经发生和神经元发育是重要的
6. Neuronal Activity-Dependent Activation of Astroglial Calcineurin in Mouse Primary Hippocampal Cultures [O] . Dmitry Lim, Lisa Mapelli, Pier Luigi Canonico, 2018

机译：小鼠原代海马培养物中神经胶质钙调神经磷酸酶的神经活动依赖激活。
7. Neurogenic and neurotrophic effects of BDNF peptides in mouse hippocampal primary neuronal cell cultures. [O] . Maria del Carmen Cardenas-Aguayo, Syed Faraz Kazim, Inge Grundke-Iqbal, 2013

机译：BDNF肽在小鼠海马原代神经细胞培养物中的神经源性和神经营养作用。

An efficient and cost-effective method of generating postnatal (P2-5) mouse primary hippocampal neuronal cultures

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