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首页> 外文期刊>Journal of Neuroscience Methods >Comparison among ultrasonic, electrical apparatus, and toxic chemicals for vestibular lesion in mice
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Comparison among ultrasonic, electrical apparatus, and toxic chemicals for vestibular lesion in mice

机译：小鼠前庭病变的超声波，电气设备和有毒化学物质的比较

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Highlights ? Vestibular lesion induced using an ultrasonicator shows complete deletion of vestibular function without non-specific damage. ? Vestibular lesion can be induced chemically in C57BL/6J but not in ICR mice. ? Body mass in the vestibular lesion group was significantly smaller than that in the control group. Abstract Background The vestibular lesion (VL) is required to examine the physiological function of the vestibular system in animals. Toxic chemicals or electrical apparatus have been used for the VL, however, they are not ideal as they have low specificity, and can result in unintended damage, and systemic toxic effect. New method Localized vibration-induced VL, using an ultrasonicator, is expected to overcome the problems associated with chemical and electrical lesions. Thus, we examined the effect of the ultrasonication on the VL from the aspects of both the physiological function and histology in the present study. Results and Comparison with Existing Method(s) Complete VL, which was evaluated by deterioration of swimming skills, righting reflex, and body stability, was induced using an ultrasonicator or electrical apparatus. Histological evaluation shows that hair cell layers in the saccule and utricle were completely destroyed in both methods Furthermore, significant drop in body mass was observed in VL. However, abscess at the cranial base was observed in VL induced by the electrical apparatus in ICR mice. Complete chemically-induced VL was observed in C57BL/6J but not ICR mice, and systemic leakage of the toxic chemicals (arsenic) was not detectable even 1day after surgery. Conclusions Compared to the electrical apparatus, the ultrasonicator is useful for inducing VL in ICR and C57BL/6J mice, as it results in less non-specific damage. Toxic chemicals can be used for inducing VL in C57BL/6J mice; however, this method does not ensure complete disruption of the hair cells in the saccule and utricle.
机译：强调 ？使用超声波器诱导的前庭病变显示出包围功能的完全缺失，没有非特定损坏。还前庭病变可以在C57BL / 6J中化学诱导，但不在ICR小鼠中诱导。还前庭病变组体重明显小于对照组的体重。摘要背景前庭病变（VL）需要检查动物中前庭系统的生理功能。有毒化学物质或电气设备已用于VL，但是，它们并不理想，因为它们具有低特异性，并且可能导致意外损坏和系统性有毒效果。预计使用超声波振动诱导VL的新方法将克服与化学和电病变相关的问题。因此，我们从本研究中的生理功能和组织学方面检查了超声波对VL的影响。结果和与现有方法的比较完成VL，通过超声波或电气设备诱导通过游泳技能的恶化，右转反射和身体稳定性进行评估。组织学评价表明，在两种方法中，囊细胞层和尿布氏菌在两种方法中完全破坏，在VL中观察到体重显着下降。然而，在ICR小鼠中的电气设备诱导的VL中观察到颅底处的脓肿。在C57BL / 6J中观察到完全化学诱导的VL，但不是ICR小鼠，并且甚至在手术后1天均未检测到有毒化学物质（砷）的全身泄漏。结论与电气设备相比，超声波器可用于在ICR和C57BL / 6J小鼠中诱导VL，因为它导致较少的非特异性损坏。有毒化学物质可用于在C57BL / 6J小鼠中诱导VL;然而，该方法不会确保囊状细胞和utricle中的毛细胞完全破坏。

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