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首页> 外文期刊>Journal of Molluscan Studies >Detection of hybrid Pyganodon grandis and P.lacustris (Bivalvia: Unionidae) using F- and M-type mitochondrial DNA sequences and geometric morphometrics
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Detection of hybrid Pyganodon grandis and P.lacustris (Bivalvia: Unionidae) using F- and M-type mitochondrial DNA sequences and geometric morphometrics

机译：使用F和M型线粒体DNA序列和几何形态学测定学检测杂交型枸杞祖氏和P.Lacustris（Bivalvia：Enionidae）

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摘要

Pyganodon grandis and P. lacustris, widespread and common species in North America, are known to co-occur across parts of their distributions. These mussels are genetically distinguishable with a sequence divergence between 9% and 13% for the mitochondrial (mt) DNA barcoding region, cytochrome c oxidase subunit I (COI), which is well beyond thresholds for distinct species. The objectives of this study were to (1) confirm the presence of two genetically distinct species (P. grandis and P. lacustri) in Font Lake and Lake Geneserath on Beaver Island in northern Lake Michigan, (2) identify hybrids using mitogenotype mismatch and (3) compare species and populations morphometrically. Of the 212 specimens sequenced, 146 (68.9%) were found to be heteroplasmic, with successful amplifications of both maternally (F-type, from mantle tissue) and paternally (M-type, from gonad tissue) inherited mt DNA sequences. Mismatches in species identity of the F- and M-type COI sequences were found in 22 specimens (15.1% of heteroplasmic specimens), with these being considered putative hybrids. Geometric morphometric analyses of shell shape reliably differentiated specimens by species (identified using COI sequences) and between lakes, correctly assigning 97.4% of homoplasmic and heteroplasmic specimens with matching mitogenomes to both species and lake of origin. The shape of putative hybrids generally resembled those of the F-type mt DNA sequence that was recovered. This study confirms that hybridization does occur between sympatric Itanodon species. Additionally, our results reemphasize the value of modern morphometric techniques for distinguishing among species and populations with overlapping morphological variability.
机译：据了解北美的Pyganodon Grandis和P. Lapustris，普遍和常见的物种，遍布其分布的各个部分。这些贻贝在线粒体（MT）DNA条形码区，细胞色素C氧化酶亚基I（COI）的9％至13％之间的序列性差异，其源于不同物种的阈值。本研究的目标是（1）确认在Michigan北部的Beaver Island上的Font Lake和Geneserath中的两种遗传Xandis和P. Lapustri）的存在，（2）使用毒催化不匹配识别杂种物（3）比较物种和人群的形式。在212种样品中测序，发现146（68.9％）是异质的，具有潜水（F型，来自披风组织）和患者（来自Gonad组织的M型）的成功扩增遗传的MT DNA序列。在22个标本（15.1％的异质标本）中发现了F-and M型COI序列的物种同一性的不匹配，其中包括据推定的杂种。壳体形状的几何形态学分析可靠地分化样品（使用COI序列鉴定）和湖泊之间，正确地分配97.4％的同源性和异质标本，其含有与原产地和湖泊匹配的毒蛛。推定的杂种的形状通常类似于回收的F型MT DNA序列的形状。该研究证实，在SympaTric Itanodon物种之间发生杂交。此外，我们的结果致力于现代形态学技术的价值，以区分物种和群体与重叠形态变异性。

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  • 来源
    《Journal of Molluscan Studies》 |2020年第3期|共7页
  • 作者

    Beauchamp Kate C.; Beyett Tyler W.; Scott Mariah W.; Zanatta David T.;

  • 作者单位

    Cent Michigan Univ Dept Biol Inst Great Lakes Res Mt Pleasant MI 48859 USA;

    Cent Michigan Univ Dept Biol Inst Great Lakes Res Mt Pleasant MI 48859 USA;

    Cent Michigan Univ Dept Biol Inst Great Lakes Res Mt Pleasant MI 48859 USA;

    Cent Michigan Univ Dept Biol Inst Great Lakes Res Mt Pleasant MI 48859 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物学;
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