• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molluscan Studies >A new set of endogenous control genes for use in quantitative real-time PCR experiments show that formin Ldia2(dex) transcripts are enriched in the early embryo of the pond snail Lymnaea stagnalis (Panpulmonata)
【24h】

A new set of endogenous control genes for use in quantitative real-time PCR experiments show that formin Ldia2(dex) transcripts are enriched in the early embryo of the pond snail Lymnaea stagnalis (Panpulmonata)

机译：用于定量实时PCR实验的一组新的内源性对照基因表明，甲状腺蛋白（DEX）转录物的含量富含池塘蜗牛Lymnaea Stagnalis（Panpulmonata）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although the pond snail Lymnaea stagnalis is an emerging model organism for molecular studies in a wide variety of fields, there are a limited number of verified endogenous control genes for use in quantitative real-time PCR. As part of a larger study on snail chirality, or left right asymmetry, we assayed gene expression in pond snail embryos. We evaluated six candidate control genes, by comparing their expression in three tissues (ovotestis, foot and embryo) and used three software programmes (geNorm, Normfinder and Bestkeeper) to do so. The specific utility of these control genes was then tested by investigating the relative expression of six experimental transcripts, including formin Ldia2, a gene that has been associated with chiral variation in L. stagnalis. All six control genes were found to be suitable for use in the three tissues tested. Of the six experimental genes, it was found that all were relatively depleted in the early embryo compared with other tissues, except the formin Ldia2 gene. Instead, transcripts of the wild-type Ldia2 were enriched in the embryo, whereas a nonfunctional frameshifted version, Ldia2, was severely depleted. These differences in Ldia2s'n expression were less evident in the ovotestis and were not evident in the foot tissue, possibly because nonsense-mediated decay is obscured in actively transcribing tissues. Our work provides a set of control genes that may be useful to the wider community and illustrates how these genes may be used to assay differences in expression in a variety of tissues.
机译：虽然池塘蜗牛Lymnaea Stagnalis是一种用于各种各样的田间的新出现的模型生物，但是有限数量的验证的内源性对照基因用于定量实时PCR。作为对蜗牛手性的较大研究的一部分，或左不对称，我们在池塘蜗牛胚胎中测定基因表达。通过将其表达与三种组织（Ovotestis，脚和胚胎）的表达进行比较，我们评估了六种候选控制基因，并使用了三个软件程序（Genorm，Normfinder和Bestkeeper）来进行。然后通过研究六个实验转录物的相对表达，包括甲蛋白LDIA2，与L. Stagnalis的手性变化相关的基因来测试这些对照基因的具体效用。发现所有六种对照基因适用于测试的三种组织中。在六种实验基因中，发现除了甲状腺素LDIA2基因外，胚胎的早期胚胎中的所有相对均匀均匀。相反，野生型LDIA2的转录物富集在胚胎中，而无功能的架空版本LDIA2被严重耗尽。在卵巢中，LDIA2S'n表达的这些差异在卵巢中不太明显，并且可能在脚部组织中不明显，可能是因为废话介导的衰减在积极转录组织中被遮挡。我们的作品提供了一组对更广泛的群落有用的对照基因，并说明这些基因如何用于测定各种组织中表达的差异。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号