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首页> 外文期刊>Journal of orthopaedic research >Transforming growth factor beta mediates communication of co-cultured human nucleus pulposus cells and mesenchymal stem cells
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Transforming growth factor beta mediates communication of co-cultured human nucleus pulposus cells and mesenchymal stem cells

机译：转化生长因子β介导共培养人核骨髓细胞和间充质干细胞的通信

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摘要

Intervertebral disc (IVD) consists of surrounding tissue annulus fibrosus and central nucleus pulposus, which are partially degenerative in scoliotic IVDs. Successful regeneration of scoliotic alterations requires cognition of critical paracrine mediators of cell-to-cell contact in the IVD. In this work, we hypothesized that transforming growth factor beta (TGF-beta) is involved in the intercellular communication of nucleus pulposus cells (NPCs) and mesenchymal stem cells (MSCs). We observed that in cultured NPCs TGF-beta 1 stimulated COL1A1 expression, encoding collagen I, and in MSCs stimulated COL1A1 and SOX9 expressions. We subsequently co-cultured NPCs and MSCs together using direct and indirect transwell systems. The expression of miR-140 and miR-145 were decreased in co-cultured NPCs. We observed that direct co-culture system stronger than the indirect system decreased expression of three miRNA. The expression of COL1A1, ACAN, encoding aggrecan, and SOX9 genes was increased in MSCs co-cultured with NPCs. Co-cultures were incubated with two inhibitors of TGF-beta type I receptor: SB-431542 and SB-525334. In co-cultured NPCs, SB-431542 and SB-525334 annulated downregulation of miR-140 and miR-145. In MSCs these inhibitors diminished stimulation of COL1A1, ACAN, and SOX9. We concluded that stimulation of COL1A1, ACAN, and SOX9 in co-cultured MSCs and regulation of miR-140 and miR-145 in NPCs were TGF-beta-dependent and TGF-beta is involved in the communication of NPCs and MSCs in co-culture. (c) 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:3023-3032, 2018.
机译：椎间盘（IVD）由周围的组织环纤维和中央核浆组成，其在微菌IVDS中是部分退行性的。成功再生的脊生改变需要认识到IVD中细胞对细胞接触的临界旁静脉介质。在这项工作中，我们假设转化生长因子β（TGF-β）参与细胞核拷贝细胞（NPC）和间充质干细胞（MSC）的细胞间通信。我们观察到，在培养的NPCS TGF-β1中刺激COL1A1表达，编码胶原I，和MSCs刺激的COL1A1和SOX9表达。随后使用直接和间接的Transwell系统将NPC和MSC共同培养。在共培养的NPC中，miR-140和miR-145的表达减少。我们观察到，直接共同培养系统比间接体系更强，减少了三个miRNA的表达。用NPC共同培养的MSCs中，COL1A1，Acan，编码骨料和SOX9基因的表达增加。将共培养物与TGF-β型受体的两种抑制剂一起温育：Sb-431542和Sb-525334。在共培养的NPC中，Sb-431542和Sb-525334在MiR-140和miR-145的下调下调。在MSCs中，这些抑制剂减少了COL1A1，ACAN和SOX9的刺激。我们得出结论，COL1A1，ACAN和SOX9在共同培养的MSC和MIR-140和MIR-145中的刺激是TGF-Beta依赖性的，TGF-β参与COPCS和TGF-Beta在CO-中的NPC和MSCs的通信文化。 （c）2018年骨科研究会。由Wiley期刊出版，Inc。J Orthop Res 36：3023-3032,2018。

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