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首页> 外文期刊>Journal of proteomics >An 'omic' approach to Pyrocystis lunula: New insights related with this bioluminescent dinoflagellate
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An 'omic' approach to Pyrocystis lunula: New insights related with this bioluminescent dinoflagellate

机译：一种“OMIC”方法对红霉狼的方法：与这种生物发光恐龙相关的新见解

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摘要

Pyrocystis lunula (Schutt) is a photoautotrophic dinoflagellate without armored form, frequently found in marine environments. Today, there are several biotechnological applications derived from the bioluminescent system of this species. From a post-genomic perspective, in order to have a starting point for studying the proteome of P. lunula, an "omics" approach (transcriptomics-proteomics) was assessed using fresh microalgae samples. A total of 80,874,825 raw reads were generated (11,292,087,505 bp; 55.82% GC) by mRNA sequencing. Very high-quality sequences were assembled into 414,295 contigs (219,203,407 bp; 55.38% GC) using Trinity software, generating a comprehensive reference transcriptome for this species. Then, a P. lunula proteome was inferred and further employed for its analysis on this species. A total of 17,461 peptides were identified, yielding 3182 protein identification hits, including 175 novel proteins. The identified proteins were further categorized according to functional description and gene ontology classification.
机译：Pyrocystis unula（Schutt）是一种没有装甲形式的光拍般的Dinoflageate，通常在海洋环境中发现。如今，有几种生物技术应用来自该物种的生物发光系统。从后基因组角度来看，为了具有研究P.Unula的蛋白质组的起点，使用新鲜的微藻样品评估“OMICS”方法（转录组织 - 蛋白质组学）。通过mRNA测序产生总共产生80,874,825个原始读取（11,292,087,505bp; 55.82％gc）。使用Trinity软件将非常高质量的序列组装成414,295个Contigs（219,203,407bp; 55.38％GC），为此物种产生综合参考转录组。然后，推断出血管蛋白质组并进一步用于分析该物种。鉴定了总共17,461个肽，产生3182个蛋白质鉴定命中，包括175个新的蛋白质。根据功能描述和基因本体分类，进一步分类所识别的蛋白质。

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