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首页> 外文期刊>Journal of Petroleum Science & Engineering >Biosurfactant production under diverse conditions by two kinds of biosurfactant-producing bacteria for microbial enhanced oil recovery
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Biosurfactant production under diverse conditions by two kinds of biosurfactant-producing bacteria for microbial enhanced oil recovery

机译：通过两种生物活性剂的微生物增强型油回收产生两种生物活性剂的生物活性剂生产

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摘要

Stable growth and efficient production of biosurfactant by functional bacteria in oil reservoirs are indispensable for MEOR process. Biosurfactant production of bacteria has been mainly studied under aerobic conditions, but information on biosurfactant production under diverse conditions is still scarce. Two kinds of common biosurfactant-producing bacteria (Bacillus amyloliquefaciens 702 and Pseudomonas aeruginosa 709) were isolated from production fluid of Xinjiang oil field, China. Biosurfactant production by two strains was investigated under aerobic conditions, anaerobic conditions and high pressure (10 MPa) conditions, respectively. Under aerobic conditions, both B. amyloliquefaciens 702 and P. aeruginosa 709 decreased the surface tension of aerobic culture to lower than 27 mN/m at temperatures (25 degrees C-40 degrees C), pH values (6.0-9.0) and less than 30 g/l NaCl. Strains 702 and 709 can aerobically produce 1582.4 mg/l and 8237.5 mg/l of biosurfactant. Both two aerobic cultures emulsified crude oil with EI24 values up to 84%. Both biosurfactant produced by strains 702 and 709 are thermostable and halo-tolerant. Although both two strains can grow under anaerobic conditions or high pressure conditions, only strain 709 efficiently produced biosurfactant under these conditions. Results of core flooding tests showed that another 7.04% of original crude oil was displaced through in situ production of biosurfactant by strain 709, and 1.10% of original crude oil was recovered by strain 702. Strain P. aeruginosa 709 can efficiently produce biosurfactant under anaerobic and high pressure conditions, so it is more suitable and advantageous for in situ MEOR applications. The feasibility of in situ production of biosurfactant used in oil fields was also discussed.
机译：油藏功能细菌的稳定增长和有效生产生物活体剂是对MEOR过程不可或缺的。生物表面活性剂的细菌主要在有氧条件下研究，但有关不同条件下的生物活性剂生产的信息仍然稀缺。从新疆油田的生产流体中分离出两种常见的生物活性剂的细菌（Bacillus淀粉醇淀粉氨酰胺嘧啶702和假单胞菌Aerginosa 709）。在有氧条件下，厌氧条件和高压（10MPa）条件下研究了两种菌株的生物活性剂的产生。在有氧条件下，B.淀粉氨酰胺702和P.铜绿假单胞菌709在温度（25摄氏度C-40℃），pH值（6.0-9.0）和小于30克/升NaCl。菌株702和709可以是有氧生产1582.4mg / L和8237.5mg / L的生物活性剂。两个有氧培养物均匀乳化原油，ei24值高达84％。由菌株702和709产生的两种生物活性剂是热稳定的和卤素。虽然两个菌株都可以在厌氧条件下或高压条件下生长，但在这些条件下只有菌株709有效地产生生物活性剂。核心泛滥试验结果表明，通过菌株709的生物活性剂的原位产生的另外7.04％的原始原油流离失所，并通过菌株702回收1.10％的原油。菌株P.铜绿假单胞菌709可以有效地在厌氧下产生生物活性剂和高压条件，因此在原位MEOR应用中更适合和有利。还讨论了石油场中使用的生物活性剂的原位生产的可行性。

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