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首页> 外文期刊>Journal of Parasitology >DEVELOPING AN APICOMPLEXAN DNA BARCODING SYSTEM TO DETECT BLOOD PARASITES OF SMALL CORAL REEF FISHES
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DEVELOPING AN APICOMPLEXAN DNA BARCODING SYSTEM TO DETECT BLOOD PARASITES OF SMALL CORAL REEF FISHES

机译：开发ApiCoMplexan DNA条形码系统以检测小珊瑚礁鱼的血液寄生虫

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摘要

Apicomplexan parasites are obligate parasites of many species of vertebrates. To date, there is very limited understanding of these parasites in the most-diverse group of vertebrates, actinopterygian fishes. While DNA barcoding targeting the eukaryotic 18S small subunit rRNA gene sequence has been useful in identifying apicomplexans in tetrapods, identification of apicomplexans infecting fishes has relied solely on morphological identification by microscopy. In this study, a DNA barcoding method was developed that targets the 18S rRNA gene primers for identifying apicomplexans parasitizing certain actinopterygian fishes. A lead primer set was selected showing no cross-reactivity to the overwhelming abundant host DNA and successfully confirmed 37 of the 41 (90.2%) microscopically verified parasitized fish blood samples analyzed in this study. Furthermore, this DNA barcoding method identified 4 additional samples that screened negative for parasitemia, suggesting this molecular method may provide improved sensitivity over morphological characterization by microscopy. In addition, this PCR screening method for fish apicomplexans, using Whatman FTA preserved DNA, was tested in efforts leading to a more simplified field collection, transport, and sample storage method as well as a streamlining sample processing important for DNA barcoding of large sample sets.
机译：Apicomplexan寄生虫是许多脊椎动物的寄生虫。迄今为止，对这些寄生虫在最多多样化的脊椎动物，Altinopterygian鱼类中非常有限的了解。虽然靶向真核细胞18s的DNA条形码是用于鉴定Tetrapods中的ApiCoMemplemer，但感染鱼类的鉴定仅仅依赖于显微镜的形态鉴定。在该研究中，开发了DNA条形码方法，其靶向18S rRNA基因引物，用于鉴定寄生物种诱导某种抗吐工性的鱼类。选择铅底漆组，显示对压倒性丰富的宿主DNA没有交叉反应性，并在本研究中分析的41（90.2％）的显微镜验证的鱼血样品中成功证实了37个。此外，该DNA条形码方法确定了4种筛选寄生虫的阴性的另外一个样品，表明该分子方法可以通过显微镜检查的形态表征提供改善的敏感性。此外，使用Whatman FTA保存DNA的鱼ApiCOMPLEN的PCR筛选方法在努力中进行了导致更简化的场收集，运输和样品储存方法以及对大型样品集的DNA条形码重要的精简样本处理。

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