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首页> 外文期刊>Journal of Shellfish Research >REOVIRUS INFECTION CHANGES TRANSCRIPT LEVELS OF EUKARYOTIC TRANSLATION INITIATION FACTOR 4E (eIF4E) FAMILY MEMBERS AND eIF4E-BINDING PROTEIN (4E-BP) IN THE BLUE CRAB CALLINECTES SAPIDUS
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REOVIRUS INFECTION CHANGES TRANSCRIPT LEVELS OF EUKARYOTIC TRANSLATION INITIATION FACTOR 4E (eIF4E) FAMILY MEMBERS AND eIF4E-BINDING PROTEIN (4E-BP) IN THE BLUE CRAB CALLINECTES SAPIDUS

机译：reovirus感染在蓝蟹胼callinectes sapidus中改变真核翻译引发因子4e（efif4e）家族成员和eif4e结合蛋白（4e-bp）的转录物水平

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The blue crab Callinectes sapidus reovirus 1 (CsRV1), a double-stranded RNA virus, belongs to the Class III virus family Reoviridae and infects marine invertebrates, including the blue crab C. sapidus. Callinectes sapidus reovirus 1 levels correlate with disease progression, and high levels of infection usually result in death. To understand CsRV1 disease progression. the impact of CsRV1 infection on the translation initiation machinery in adult female C. sapidus was determined. Using transcriptome analysis of C. sapidus, sequences representing each of the three classes of metazoan eukaryotic translation initiation factor 4E (eIF4E) family members, eIF4E1, eIF4E2, and eIF4E3, as well as the eIF4E-binding protein (4E-BP), were identified as the first description of a Class III eIF4E in decapod crustaceans. Transcripts encoding the three eIF4E family members and 4E-BP are found in all tissues examined by end-point RT-PCR analysis, except for the hypodermis. In the noninfected animals, EIF4E1 transcript levels are similar to 11.5- to 150-fold higher than those of EIF4E2, EIF4E3, or 4EBP in both hemocytes and eyestalk ganglia. In CsRV1 infected animals, transcript levels of eIF4E family members and 4EBP initially increase in hemocytes and to a much lesser extent in eyestalk ganglia. In hemocytes, EIF4E2, EIF4E3, and 4EBP transcript levels arc highest at low levels of infection. By contrast, ElF4E1 transcript levels peak at moderate levels of infection. In the eyestalk ganglia, an endocrine tissue, CsRV1 infection only slightly impacts ELF4E1 and EIF4E2 levels, although more substantial increases in EIF4E3 and 4EBP are seen. These results provide the first window into how CsRV1 infection and disease progression perturb the host C. sapidus eIF4E family members and 4E-BP in different tissues, suggesting nuanced responses of the host translational machinery during disease progression.
机译：蓝蟹CallOxectessaapidus reovirus 1（CSRv1），一种双链RNA病毒属于III类病毒家族reoviridae，并感染海洋无脊椎动物，包括蓝蟹C. Sapidus。 Calloinectes Sapidus Retovirus 1水平与疾病进展相关，并且高水平的感染通常导致死亡。了解CSRV1疾病进展。确定了CSRV1感染对成年女性C. Sapidus的翻译起始机械的影响。使用C. sapidus的转录组分析，表示三类三类真核形态翻译引发因子4e（EIF4E）家族成员，EIF4E1，EIF4E2和EIF4E3以及eIF4E结合蛋白（4E-BP）的序列是鉴定为脱像甲壳类动物III类EIF4E的第一个描述。在通过终点RT-PCR分析检查的所有组织中发现编码三个EIF4E系列成员和4E-BP的转录物，除了皮下注射。在非染色的动物中，EIF4E1转录物水平与血细胞和眼睑神经细胞和眼部的EIF4E2，EIF4E3或4EBP相似的11.5至150倍。在CSRv1受感染的动物中，EIF4E家族成员的转录水平和4EBP最初在血细胞增加并在眼睑神经节的程度上更大程度地增加。在血细胞，EIF4E2，EIF4E3和4EBP转录水平在低水平的感染时最高。相比之下，ELF4E1转录水平在中等感染水平下的峰。在眼部组织中，内分泌组织，CSRV1感染仅略微影响ELF4E1和EIF4E2水平，尽管EIF4E3和4EBP中的更大幅增加。这些结果提供了第一个窗口进入CSRV1感染和疾病进展如何扰乱不同组织中的宿主C.Sapidus eIF4E家族成员和4E-BP，暗示疾病进展过程中宿主翻译机制的细致响应。

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