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首页> 外文期刊>Journal of stroke and cerebrovascular diseases: The official journal of National Stroke Association >Intravenous Transplants of Human Adipose-Derived Stem Cell Protect the Rat Brain From Ischemia-Induced Damage
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Intravenous Transplants of Human Adipose-Derived Stem Cell Protect the Rat Brain From Ischemia-Induced Damage

机译：人脂肪衍生的干细胞的静脉内移植保护大鼠脑免受缺血诱导的损伤

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摘要

Background: Survival following cardiac arrest (CA) and subsequent cardiopulmonary resuscitation (CPR), to a great extent, depends on brain damage. Adipose-derived stem cells (ADSCs), as a source of paracrine growth factors and the capacity of neural differentiation may reduce this brain damage. Objective: The purpose of this study is to evaluate the protection of ADSCs to brain damage following CPR. Methods: Rats were divided into 3 groups, sham, CA, and ADSCs group. Rats in sham group went through sham surgery. Rats in CA group went through CA, CPR, and injection PBS (phosphate buffer saline). Rats in ADSCs group went through CA, CPR, and intravenous injection of ADSCs. Rats in sham group were sacrificed immediately after operation. At 24, 72, and 168 hours after return of spontaneous circulation operation, rats in CA and ADSCs group were randomly selected and sacrificed. Brain damage was evaluated by using Neurological Deficit Scale (NDS) score, hippocampal pathology, serum level of S100 beta, and apoptosis ratio of hippocampal neurons. Protein of brain derived neurotrophic factor (BDNF) and IL-6 (interleukin-6) in the hippocampus were detected. Results: Compared with sham group, CA and ADSCs group showed a decrease in NDS score, an increased apoptosis ratio of hippocampal nerve cells, increased serum level of S100-beta, and a significant increase in neuroprotective IL-6 and BDNF. In comparison to CA group, ADSCs group had a mild degree of brain damage and higher expression of IL-6 and BDNF. Conclusions: In the acute stage of cerebral injury following CA, ADSCs might improve the prognosis of brain damage by stimulating the expression of neuroprotective IL-6 and BDNF.
机译：背景：心脏骤停（CA）和随后的心肺复苏（CPR）后生存在很大程度上取决于脑损伤。脂肪衍生的干细胞（ADSCs），作为旁静脉生长因子的来源和神经分化的能力可能降低这种脑损伤。目的：本研究的目的是评估CPR后脑损伤的ADSC的保护。方法：将大鼠分为3组，假，CA和ADSCS组。虚假小组的大鼠经历了假手术。 CA组中的大鼠通过Ca，CPR和注射PBS（磷酸盐缓冲盐）。 ADSCS组大鼠通过CA，CPR和静脉注射ADSC。手术后立即处死假手术大鼠。在自发循环操作返回后的24,72和168小时，随机选择和处死CA和ADSC组中的大鼠。通过使用神经缺陷量表（NDS）得分，海马病理学，S100β的血清水平和海马神经元的凋亡率来评估脑损伤。检测脑衍生的神经营养因子（BDNF）和IL-6（白细胞介素-6）的蛋白质被检测到海马中。结果：与假手术组相比，CA和ADSCS组显示NDS评分的降低，海马神经细胞的凋亡率增加，血清S100-β的血清水平增加，并且神经保护IL-6和BDNF的显着增加。与CA组相比，ADSCS组具有轻度的脑损伤程度和更高的IL-6和BDNF表达。结论：在CA后脑损伤的急性阶段，ADSCs通过刺激神经保护IL-6和BDNF的表达来改善脑损伤的预后。

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