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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Bio-inspired solute enables preservation of human oocytes using minimum volume vitrification
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Bio-inspired solute enables preservation of human oocytes using minimum volume vitrification

机译：生物启发溶质能够使用最小体积玻璃化保存人卵母细胞

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摘要

The ability to cryopreserve human oocytes has significant potential for fertility preservation. Current cryopreservation methods still suffer from the use of conventional cryoprotectants, such as dimethyl sulphoxide (DMSO), causing loss of viability and function. Such injuries result from the toxicity and high concentration of cryoprotectants, as well as mechanical damage of cells due to ice crystal formation during the cooling and rewarming processes. Here we report the preservation of human oocytes following vitrification using an innovative bio-inspired cryoprotectant integrated with a minimum volume vitrification approach. The results demonstrate that the recovered human oocytes maintained viability following vitrification and rewarming. Moreover, when this approach was used to vitrify mouse oocytes, the recovered oocytes preserved their viability and function following vitrification and rewarming. This bio-inspired approach substitutes DMSO, a well-known toxic cryoprotectant, with ectoine, a non-toxic naturally occurring solute. The bio-inspired vitrification approach has the potential to improve fertility preservation for women undergoing cancer treatment and endangered mammal species.
机译：冷冻保存人类卵母细胞的能力具有巨大的生育保存潜力。电流冷冻保存方法仍然患有常规冷冻保护剂，例如二甲基亚砜（DMSO），导致活力损失和功能。这种损伤是由冷冻保护剂的毒性和高浓度导致的，以及由于在冷却和复活过程中由于冰晶形成而导致细胞的机械损伤。在这里，我们通过与最小体积玻璃化方法集成的创新生物启发冷冻保护剂报告玻璃化后的人卵母细胞的保存。结果表明，回收的人卵母细胞在玻璃化和复活后保持活力。此外，当这种方法用于玻璃化小鼠卵母细胞时，回收的卵母细胞在玻璃化和复活后保留了它们的活力和功能。这种生物启发方法替代DMSO，一种众所周知的有毒冷冻保护剂，卵巢，一种无毒的天然存在的溶质。生物启发的玻璃化方法有可能改善癌症治疗和濒危哺乳动物物种的妇女的生育保存。

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