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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement
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Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement

机译：脱细胞牛腺细胞可以作为具有保存的血管布置的生物支架

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Technically produced scaffolds are common to establish transplantable tissues for regenerative medicine, but also biological ones that are closer to the natural condition become of interest. Placentas are promising, because they represented available, complete organs with rich extracellular matrix (ECM) and well-developed vasculature that easily could build anastomoses to a host's organ. Only placentas from larger animal models such as the bovine meet the dimensions large enough for most organs but are not adequately described yet. We here studied the nature of the ECM in 27 natural and decellularized bovine cotyledons, that is, the fetal part of the placentomes, by means of histology, immunohistochemistry, and electron microscopy. Successful decellularization was done by perfusion with 0.01%, 0.1%, and 0.5% sodium dodecyl sulfate each and subsequent immersion in 1% Triton X-100, resulting in a removal of cells and DNA, whereas the structure of the allantochorionic surface and villi was preserved. Although some fibres disappeared, also the arrangement of the main ECM proteins was largely similar before and after decellularization: Along the larger vessels, a densely packed network of thick fibres occurred, organized in layers without cells or spaces in between. Collagen IV, fibronectin, and laminin contributed to those areas. In contrast, collagen I and III characterized the meshwork of medium-sized and thin fibres in the mesenchyme, respectively. In conclusion, decellularized bovine cotyledons indeed had characteristics of a biological scaffold and provide an interesting alternative to develop large-scale scaffolds with complex vascular architecture for tissue engineering purposes.
机译：技术生产的支架是常见的，用于建立再生医学的可移植组织，也是更接近自然状况的生物学的组织。胎盘很有希望，因为它们代表了可用的，具有富含细胞外基质（ECM）的完整器官，以及良好的血管系统，容易将吻合吻合给主机的器官。只有来自较大的动物模型的胎盘，例如牛的尺寸足够大的尺寸，但尚未充分描述。我们在这里研究了27种天然和脱细胞牛牛腔内的ECM的性质，即胎盘的胎儿部分，通过组织学，免疫组织化学和电子显微镜。通过灌注成功的脱细胞化通过灌注0.01％，0.1％和0.5％十二烷基硫酸钠，然后在1％Triton X-100中浸泡，导致细胞和DNA的去除，而掩盖化学表面和绒毛的结构保存。虽然一些纤维消失了，但在脱细胞化之前和之后，主要ECM蛋白的布置也在很大程度上类似：沿着较大的血管，发生在没有细胞或之间的层或空间的层中发生密集的厚纤维网络。胶原蛋白IV，纤连蛋白和层粘连蛋白贡献给这些区域。相反，胶原蛋白I和III分别表征了间充质中的中尺寸和薄纤维的网状。总之，脱细胞牛胞嘧啶确实具有生物支架的特点，并提供了一种有趣的替代方案，可以开发具有复杂血管架构的大规模支架，用于组织工程目的。

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