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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Design and biological functionality of a novel hybrid Ti-6Al-4V/hydrogel system for reconstruction of bone defects
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Design and biological functionality of a novel hybrid Ti-6Al-4V/hydrogel system for reconstruction of bone defects

机译：新型杂交Ti-6AL-4V /水凝胶系统的设计和生物学功能，用于重建骨缺损

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We have designed a unique injectable bioactive hydrogel comprising of alginate, gelatin, and nanocrystalline hydroxyapatite and loaded with osteoblasts, with the ability to infiltrate into three-dimensional Ti-6Al-4V scaffolds with interconnected porous architecture, fabricated by electron beam melting. A two-step crosslinking process using the EDC/NHS and CaCl2 was adopted and found to be effective in the fabrication of cell-loaded hydrogel/Ti-6Al-4V scaffold system. This hybrid Ti-6Al-4V scaffold/hydrogel system was designed for the reconstruction of bone defects, which are difficult to heal in the absence of suitable support materials. The hybrid Ti-6Al-4V/hydrogel system favourably modulated the biological functions, namely, adhesion, proliferation, cell-to-cell, and cell-material communication because of the presence of extracellular matrix-like hydrogel in the interconnected porous structure of 3D printed Ti-6Al-4V scaffold. The hydrogel was cytocompatible, which was proven through live/dead assay, the expression level of prominent proteins for cell adhesion and cytoskeleton, including 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Furthermore, the high bone formation ability of the hydrogel was confirmed using alkaline phosphatase assay. A high equilibrium water content (similar to 97%) in the hydrogel enables the delivery of cells and bioactive molecules, necessary for bone tissue growth. Although not studied, the presence of hydrogel in the pores of the scaffold can provide the space for the cell migration as well as vascularization through it, required for the effective exchange of nutrients. In conclusion, we underscore that the 3D-printed Ti-6Al-4V scaffold-loaded with bioactive hydrogel to treat the bone defects significantly impacted cellular functions and cell-material interaction.
机译：我们设计了一种独特的可注射生物活性水凝胶，其包含藻酸盐，明胶和纳米晶体羟基磷灰石，并用成骨细胞装载，具有渗透到三维Ti-6Al-4V支架中，具有通过电子束熔化制造的互连的多孔结构。采用了使用EDC / NHS和CACL2的两步交联过程，发现在制备电池加载的水凝胶/ Ti-6Al-4V支架系统中是有效的。这种混合TI-6AL-4V支架/水凝胶系统设计用于重建骨缺损，这难以在没有合适的载体材料的情况下愈合。杂交Ti-6Al-4V / Hydrogel系统有利地调节生物功能，即粘附，增殖，细胞对细胞和细胞材料通信，因为存在于3D的相互连接的多孔结构中的细胞外基质样水凝胶印刷TI-6AL-4V脚手架。水凝胶是细胞蛋白的，其通过活/死导被证明，细胞粘附和细胞骨架的突出蛋白质的表达水平，包括3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑鎓溴（MTT）测定。此外，使用碱性磷酸酶测定证实了水凝胶的高骨形成能力。水凝胶中的高均衡水含量（类似于97％）使得能够递送细胞和生物活性分子，所述骨组织生长所需的必需品。虽然未研究，但是在支架的孔中存在水凝胶可以为细胞迁移的空间提供有效交换营养物质所需的细胞迁移以及血管化。总之，我们强调了3D印刷的Ti-6Al-4V支架载有生物活性水凝胶，以治疗骨缺陷显着影响细胞功能和细胞材料相互作用。

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