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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Testing a novel nanofibre scaffold for utility in bone tissue regeneration
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Testing a novel nanofibre scaffold for utility in bone tissue regeneration

机译：测试新型纳米纤维支架在骨组织再生中的效用

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Many variables serve to alter the process of bone remodelling and diminish regeneration including the size and nature of the wound bed and health status of the individual. To overcome these inhibitory factors, tissue-engineered osteoconductive scaffolds paired with various growth factors have been utilized clinically. However, many limitations still remain, for example, bone morphogenetic protein 2 (BMP2) can lead to rampant inflammation, ectopic bone formation, and graft failure. Here, we studied the ability for a nanofiber scaffold (Talymed) to accelerate BMP2 growth factor-induced bone healing compared with the traditional absorbable collagen sponge (ACS) delivery system. One hundred fifty-five adult wild type mice were arranged in 16 groups by time, 4 and 8weeks, and treatment, ACS or Talymed, loaded with control, low, medium, or high dosages of BMP2. Skulls were subjected to microCT, biomechanical, and histological analysis to assess bone regeneration. The use of Talymed within the defect site was found to decrease the bone volume, bone formation rate, and alkaline phosphatase activity compared with ACS/BMP2 combinations. Interestingly, though Talymed regenerated less bone, the regenerate was found to have a greater hardness value than that of bone within the ACS groups. However, the difference in bone hardness between scaffolds was not detectable by 8weeks. Based on these results, we found that the nanofiber scaffold generated a better quality of bone regenerate at 4weeks but, due to the lack of overall bone formation and the inhibition of normal remodelling processes, was not as efficacious as the current clinical standard ACS/BMP2 therapy.
机译：许多变量用于改变骨重塑过程和减少再生，包括伤口床的尺寸和性质和个体的健康状况。为了克服这些抑制因素，临床上使用了与各种生长因子配对的组织工程骨导电支架。然而，许多限制仍然存在，例如，骨形态发生蛋白2（BMP2）可以导致炎症猖獗，异位骨形成和移植物失效。在这里，我们研究了与传统的吸收胶原海绵（ACS）输送系统相比加速BMP2生长因子诱导的骨愈合的能力。一百五十五个成人野生型小鼠按时间，4和8周为16组安排，治疗，ACS或算法，加载控制，低，培养基或BMP2的高剂量。对颅骨进行微生物，生物力学和组织学分析，以评估骨再生。与ACS / BMP2组合相比，发现使用缺陷部位内的差款降低骨体积，骨形成率和碱性磷酸酶活性。有趣的是，虽然差款再生较低的骨骼，发现再生具有比ACS组内的骨骼更大的硬度值。然而，在8周内，支架之间的骨骼硬度之间的差异是不可检测的。基于这些结果，我们发现纳米纤维支架在4周产生更好的骨骼质量，但由于缺乏整体骨形成和对正常重塑过程的抑制，并不像目前临床标准ACS / BMP2那么有效治疗。

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