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首页> 外文期刊>Journal of Veterinary Research >Detection of Actinobacillus pleuropneumoniae ApxIV toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions
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Detection of Actinobacillus pleuropneumoniae ApxIV toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions

机译：在实验条件下接种猪血清和口服液样本中Actinobacillus胸膜蛋白抗体的检测

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Introduction: The prevention and control of Actinobacillus pleuropneumoniae in commercial production settings is based on serological monitoring. Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect specific antibodies against a variety of A. pleuropneumoniae antigens, including long-chain lipopolysaccharides (LPS) and the ApxIV toxin, a repeats in -toxin (RTX) exotoxin unique to A. pleuropneumoniae and produced by all serovars. The objective of this study was to describe ApxIV antibody responses in serum and oral fluid of pigs. Material and Methods: Four groups of pigs (six pigs per group) were inoculated with A. pleuropneumoniae serovars 1, 5, 7, or 12. Weekly serum samples and daily oral fluid samples were collected from individual pigs for 56 days post inoculation (DPI) and tested by LPS and ApxIV ELISAs. The ApxIV ELISA was run in three formats to detect immunlgobulins M, G, and A (IgM, IgG and IgA) while the LPS ELISA detected only IgG. Results: All pigs inoculated with A. pleuropneumoniae serovars 1 and 7 were LPS ELISA serum antibody positive from DPI 14 to 56. A transient and weak LPS ELISA antibody response was observed in pigs inoculated with serovar 5 and a single antibody positive pig was observed in serovar 12 at >= 35 DPI. Notably, ApxIV serum and oral fluid antibody responses in pig inoculated with serovars 1 and 7 reflected the patterns observed for LPS antibody, albeit with a 14 to 21 day delay. Conclusion: This work suggests that ELISAs based on ApxIV antibody detection in oral fluid samples could be effective in population monitoring for A. pleuropneumoniae.
机译：介绍：商业生产环境中Actinobacillus胸膜内肺炎的预防和控制是基于血清学监控。已经开发了酶联免疫吸附测定（ELISA）以检测针对各种A.Pleuropneumoniae抗原的特异性抗体，包括长链脂多糖（LPS）和APXIV毒素，其在-Toxin（RTX）外毒素中的重复。所有塞洛维拉的胸膜炎和生产。本研究的目的是描述血清中APXIV抗体应答猪和口服液。材料和方法：用A.Pleuropneumoniae Serovars 1,5,7或12种接种四组猪（每组六猪）。每周血清样品和每日口服流体样品从个体猪接种后56天（DPI ）由LPS和APXIV ELISAS测试。 APXIV ELISA以三种形式运行，以检测免疫棉花M，G和A（IgM，IgG和IgA），而LPS ELISA仅检测到IgG。结果：接种用A.胸膜血管血清血清血清抗体的所有猪从DPI 14〜56阳性。在接种随机5的猪中观察到瞬时和弱的LPS ELISA抗体反应，并观察到单一抗体阳性猪Serovar 12在> = 35 dpi。值得注意的是，APXIV血清和口服液抗体在接种与纱洛1和7的猪中反映了对LPS抗体观察到的图案，尽管延迟14至21天。结论：这项工作表明，基于APXIV抗体检测的口腔流体样品的ELISA可以有效地对A.Pleuropneumone的人口监测有效。

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