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首页> 外文期刊>Journal of Veterinary Research >Metabolic activity of boar semen stored in different extenders supplemented with ostrich egg yolk lipoproteins
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Metabolic activity of boar semen stored in different extenders supplemented with ostrich egg yolk lipoproteins

机译：储屋精液的代谢活动储存在补充鸵鸟蛋黄脂蛋白的不同增量剂中

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Introduction: The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures. Material and Methods: Boar ejaculates were extended in Androhep, Beltsville thawing solution (BTS), and Martin-Rillo and Alias (MR-A) without (control) or with the addition of LPFo and stored for three days at either 5 degrees C or 16 degrees C. The analysed sperm parameters included total motility (TMOT), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), oxygen consumption, and adenosine triphosphate (ATP) production. Results: The sperm metabolic activity seemed to be higher in the LPFo-based extenders following storage for three days, irrespective of the storage temperature. Compared with the LPFo-free extenders, significantly higher (P < 0.05) sperm PMI and MMP were observed in BTS and MR-A extenders supplemented with LPFo during storage for three days at 5 degrees C. Spermatozoa stored in the BTS-LPFo extender exhibited higher (P < 0.05) TMOT and oxygen consumption, whereas higher (P < 0.05) PMI was observed in spermatozoa stored in Androhep-LPFo and MR-A-LPFo for three days at 16 degrees C. No significant differences (P > 0.05) in ATP content were observed between the LPFo-free and LPFo-based extenders during storage. Conclusions: Supplementation of LPFo to semen extenders had varying effects on the metabolic activity of boar spermatozoa stored at different temperatures. It can be suggested that the interactions of various components of the extenders and seminal plasma with LPFo exert beneficial effects on the sperm metabolic activity during liquid storage of boar semen.
机译：介绍：本研究的目的是评估从鸵鸟蛋黄（LPFO）分离的脂蛋白部分对液体精液在不同扩增剂和温度下的液体精液后的代谢活性的影响。材料和方法：野猪射精延伸，在贝尔茨维尔解冻溶液（BTS）和Martin-Rillo和别名（MR-A）中延伸，没有（对照）或添加LPFO并在5摄氏度或5℃或5℃下储存三天16℃。分析的精子参数包括总运动（TMOT），质膜完整性（PMI），线粒体膜电位（MMP），氧气消耗和三磷酸腺苷（ATP）生产。结果：无论储存温度如何，在储存后，LPFO的增量剂似乎在储存后似乎更高。与可LPFO的增量剂相比，在BTS和MR-MR-MR-MR-MR-MR-A在储存期间在储存期间储存三天的储存时，储存在BTS-LPFO扩展器中的SPOMATOA在BTS-LPFO扩展器中储存三天的增量剂的显着较高（P <0.05）的增量剂和MMP。较高（P <0.05）TMOT和氧气消耗，而在储存在Androhep-LPFO和MR-A-LPFO的精子中观察到更高（P <0.05）PMI，在16摄氏度下进行三天。没有显着差异（P> 0.05）在储存期间在LPFO和LPFO基的增量剂之间观察到ATP含量。结论：将LPFO与Semen Expraders的补充对储存在不同温度下的野猪精子的代谢活性不同。可以提出，随着LPFO的各种组分的相互作用与LPFO对猪精液储存期间的精子代谢活性产生有益效果。

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