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首页> 外文期刊>Journal of Veterinary Research >Apoptosis in natural rabies virus infection in dogs
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Apoptosis in natural rabies virus infection in dogs

机译：狗的天然狂犬病病毒感染的凋亡

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Introduction: In the present study apoptosis was investigated in the cornu ammonis and cerebellum of 10 dogs naturally infected with rabies virus. Diagnosis of rabies was based on the results of fluorescent antibody staining and experimental inoculation. Material and Methods: The paraffin tissue sections were stained with haematoxylin and eosin, avidin-biotin complex peroxidase (ABC-P), and terminal deoxynucleotidyl transferase biotin-dUTP nick end-labelling (TUNEL) methods. Results: Histopathological examination revealed encephalomyelitis of varying severity and the presence of Negri bodies. Dense rabies antigens were determined in the motor neurons with ABC-P method. On the other hand, Bcl-2 protein and Box protein gave positive reaction in seven and five cases, respectively. TUNEL staining demonstrated very marked apoptotic changes in the nuclei of neurons localised deep in the substantia alba of the cerebellum. Similar changes were also determined in perivascular mononuclear cells and glia cells within the substantia alba. No apoptopic changes were found in the motor neurons of the cornu ammonis. Conclusion: The absence of apoptotic changes in the neurons was considered to be the consequence of the necrotic changes that developed in these neurons.
机译：介绍：在目前的研究中，在玉米氨和10只狗的Cornu Ammonis和Cerebellum中进行了凋亡，天然感染狂犬病病毒。狂犬病的诊断是基于荧光抗体染色的结果和实验性接种。材料和方法：将石蜡组织切片用氧毒素和曙红染色，抗嘌呤 - 生物素复合过氧化物酶（ABC-P），和末端脱氧核苷酸转移酶生物素-UTP缺口末端标记（TUNEL）方法。结果：组织病理学检查揭示了不同严重程度和内格里体的存在的脑肌炎。用ABC-P方法在电动机神经元中测定密集的狂犬病抗原。另一方面，Bcl-2蛋白和盒子蛋白分别在七和五种情况下进行阳性反应。 TUNEL染色在细胞内部植物症的神经元核心内核心的核细胞核中表现出非常明显的凋亡变化。在Imageia Alba内的大脑异常单核细胞和胶质细胞中也确定了类似的变化。在Cornu Ammonis的电机神经元中没有发现凋亡性变化。结论：神经元没有凋亡变化被认为是这些神经元中发展的坏死变化的结果。

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