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首页> 外文期刊>Journal of Veterinary Research >Anti-CyHV-3 effect of fluorescent, tricyclic derivative of acyclovir 6-(4-MeOPh)-TACV in vitro
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Anti-CyHV-3 effect of fluorescent, tricyclic derivative of acyclovir 6-(4-MeOPh)-TACV in vitro

机译：荧光，Acyclovir 6-（4-Meoph）-tacv的荧光，三环衍生物的抗Cyhv-3效应

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Introduction: Cyprinid herpesvirus 3 (CyHV-3) is a virus infecting carp with disease symptoms of gill necrosis, fish discoloration, sunken eyes, and mortality reaching 90%. Several research groups have examined how to potentially abate the consequences of viral activity. Recently we showed that acyclovir inhibits CyHV-3 replication in vitro and in the present study we examined the anti-CyHV-3 activity of the tricyclic derivative of acyclovir 6-(4-MeOPh)-TACV (T-ACV), a fluorescent molecule known for higher lipophilicity than acyclovir, and therefore potentially better candidate for application in vivo. Material and Methods: CCB and KF1 cell lines were incubated with T-ACV at concentrations of 0, 66.67, and 133.33 mu M for three days and toxicity examined with MTT and CV assays. To investigate the antiviral activity of T-ACV, the lines were infected with CyHV-3 or mock infected and incubated for three days with the drug at concentrations of 0 or 66.67 mu M. The activity of T-ACV was evaluated by plaque assay and TaqMan qPCR. Results: T-ACV at a concentration of 66.67 mu M displayed low toxicity and inhibited CyHV-3 activity by 13-29%, varying by cell line and method. Conclusion: The low anti-CyHV-3 activity of T-ACV indicates that it would be reasonable to screen several tricyclic derivatives of acyclovir for such activity.
机译：介绍：Cyprinid Herpesvirus 3（Cyhv-3）是一种感染鲤鱼的病毒，病毒症状鳃坏死，鱼鳞，沉没的眼睛和死亡率达到90％。几个研究组研究了如何潜在削弱病毒活动的后果。最近，我们表明Acyclovir在体外抑制CyHV-3复制，并且在本研究中，我们检查了Acyclovir 6-（4-Meoph）-TacV（T-ACV）的三环衍生物的抗CyHv-3活性，荧光分子已知比Acyclovir更高的亲脂性，因此潜在的候选者在体内申请。材料和方法：将CCB和KF1细胞系在0,66.67和133.33μm的浓度下与T-ACV一起温育三天，并用MTT和CV测定检查毒性。为了研究T-ACV的抗病毒活性，用CyHV-3或仔细感染的线条感染并在浓度为0或66.67μm的药物中孵育三天。通过斑块测定法评价T-ACV的活性和塔克曼QPCR。结果：浓度为66.67μm的T-ACV显示出低毒性并抑制CyHV-3活性13-29％，通过细胞系和方法改变。结论：T-ACV的低抗CYHV-3活性表明，筛选ACYCLOVIR的几种三环衍生物进行此类活动是合理的。

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