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首页> 外文期刊>Journal of Veterinary Research >Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey
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Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey

机译：毒素SPP的遗传分析。 在土耳其东部的弗雷猫和狗

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Introduction: Toxocara canis and Toxocara cati are roundworms of dogs and cats. The purpose of this study was to investigate the infection caused by these ascarids in cats and dogs, using microscopic and molecular analysis methods. Material and Methods: Adult ascarids were gathered from the faeces of dogs and cats in Van province, in 2015-2016. Existing keys and PCR sequencing of the ITS-2 fragment were used to identify the morphological features of the parasite species. Results: It was observed that out of 20 adult ascarids, 17 and 3 were found to be Toxocara canis and Toxocara cati, respectively. The ITS-2 gene region was amplified by PCR to perform molecular analysis. Genotyping indicated that the dogs and cats were infected with T. canis and T. cati, respectively, and none had Toxascaris leonina. Conclusion: To the best of our knowledge, this is the first report on the molecular characteristics of adult ascaridoid nematodes from cats and dogs in Turkey. The molecular approaches established in this study enable molecular identification and genetic structure studies of the ascaridoids.
机译：简介：Toxocara Canis和Toxocara Cati是狗和猫的蛔虫。本研究的目的是使用微观和分子分析方法来研究由猫和狗的这些蛔虫引起的感染。材料和方法：2015 - 2016年，从van省的狗和猫的粪便聚集成人蛔虫。其-2片段的现有键和PCR测序用于鉴定寄生虫物种的形态学特征。结果：观察到，在20种成年蛔虫中，分别发现17和3分别是毒素Canis和Toxocara Cati。通过PCR扩增其-2基因区域以进行分子分析。基因分型表明，狗和猫分别感染了Canis和T. Cati，没有Toxascaris Leonina。结论：据我们所知，这是第一份关于土耳其猫和狗成人蛔虫线虫的分子特征的报告。本研究中建立的分子方法使得蛔虫的分子鉴定和遗传结构研究。

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