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首页> 外文期刊>Journal of Veterinary Research >Evaluation of long-term antibody response and cross-serotype reaction in ducks immunised with recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN
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Evaluation of long-term antibody response and cross-serotype reaction in ducks immunised with recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN

机译：用重组利伯拉氏菌蛋白A和CPG ODN免疫的鸭子长期抗体反应和肾脏血清型反应的评价

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Introduction: Riemerella anatipestifer (RA) infections can lead to high mortality in ducklings. Inactivated vaccines against RA are commercially available, but they fail to provide cross-protection against various serotypes. We have previously demonstrated that a subunit vaccine containing recombinant outer membrane protein A (rOmpA) antigen of serotype 2 formulated with CpG oligodeoxynucleotides (ODN) as the adjuvant was able to stimulate both humoral and cellular immunities. Material and Methods: In the present study, thirty healthy 7-day-old Pekin ducks were randomly assigned to three equal treatment groups: rOmpA-vaccinated, rOmpA + CpG-vaccinated, and control. Vaccine was injected intramuscularly and a booster dose of the same vaccine was given two weeks after primary immunisation. The long-term antibody response and cross-serotype reaction of this vaccine were evaluated in ducks. Results: Compared to ducks immunised with rOmpA alone, ducks immunised with rOmpA + CpG ODN had significantly (p < 0.05) increased serum antibody titre from two weeks until nine months after primary immunisation. In addition, expression of cytokines including interferon (IFN)-alpha, IFN-gamma, interleukin (IL)-6, and IL-12 was significantly (p < 0.05) enhanced in PBMC of ducks immunised with rOmpA + CpG ODN two weeks after primary immunisation. Antibodies from ducks immunised with the rOmpA + CpG ODN vaccine could also detect RA serotypes 1 and 6 in Western blot analysis. Conclusion: Combination of rOmpA and CpG ODN could be a feasible strategy for developing a subunit RA vaccine with long term and broader-ranging protection.
机译：简介：Riemerella Anatipestifer（RA）感染可能导致鸭子的高死亡率。对抗RA的灭活疫苗是商业上可用的，但它们未能提供针对各种血清型的交叉保护。我们之前表明，作为佐剂的含有CpG oligodeN核苷酸（ODN）配制的亚单组织外膜蛋白A（ROMPA）抗原作为佐剂，能够刺激体液和细胞免疫。材料和方法：在本研究中，三十个健康的7日龄皮鸭被随机分配给三个相等的治疗组：ROMPA接种，ROMPA + CPG接种和控制。肌肉注射疫苗，初级免疫后两周给出相同疫苗的增强剂量。在鸭子中评估该疫苗的长期抗体反应和交叉血清型反应。结果：与单独用ROMPA免疫的鸭子相比，用Rompa + CpG ODN免疫的鸭子显着（P <0.05）从两周后增加血清抗体滴度，直到初次免疫后九个月。此外，包括干扰素（IFN） - alpha，IFN-gamma，白细胞介素（IL-12和IL-12）的细胞因子的表达显着（p <0.05），在两周后与ROMPA + CPG ODN免疫的PBMC中增强原发性免疫。用ROMPA + CPG ODN疫苗免疫鸭子的抗体也可以检测Western印迹分析中的Ra血清型1和6。结论：ROMPA和CPG ODN的组合可能是一种具有长期和更广泛保护的亚基RA疫苗的可行策略。

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