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首页> 外文期刊>Journal of the Association for Research in Otolaryngology: JARO >Transplantation of mouse embryonic stem cells into the cochlea of an auditory-neuropathy animal model: effects of timing after injury.
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Transplantation of mouse embryonic stem cells into the cochlea of an auditory-neuropathy animal model: effects of timing after injury.

机译：将小鼠胚胎干细胞移植到听觉神经病变动物模型的耳蜗中：损伤后时序的影响。

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摘要

Application of ouabain to the round window membrane of the gerbil selectively induces the death of most spiral ganglion neurons and thus provides an excellent model for investigating the survival and differentiation of embryonic stem cells (ESCs) introduced into the inner ear. In this study, mouse ESCs were pretreated with a neural-induction protocol and transplanted into Rosenthal's canal (RC), perilymph, or endolymph of Mongolian gerbils either 1-3 days (early post-injury transplant group) or 7 days or longer (late post-injury transplant group) after ouabain injury. Overall, ESC survival in RC and perilymphatic spaces was significantly greater in the early post-injury microenvironment as compared to the later post-injury condition. Viable clusters of ESCs within RC and perilymphatic spaces appeared to be associated with neovascularization in the early post-injury group. A small number of ESCs transplanted within RC stained for mature neuronal or glial cell markers. ESCs introduced into perilymph survived in several locations, but most differentiated into glia-like cells. ESCs transplanted into endolymph survived poorly if at all. These experiments demonstrate that there is an optimal time window for engraftment and survival of ESCs that occurs in the early post-injury period.
机译：瓦巴恩在沙鼠的圆形窗膜中的应用选择性地诱导大多数螺旋神经节神经元的死亡，从而为研究引入内耳的胚胎干细胞的存活和分化提供了优异的模型。在这项研究中，用神经感应方案预处理小鼠ESC，并将蒙古拉伯（RC），漂白米，或蒙古·格尔伯尔的内秋氏虫（早期后损伤后移植组）或7天或更长损伤后移植组）在威达伤害后。总体而言，与后期损伤后病症的早期损伤后的微环境中，RC和漂样空间中的Esc生存率明显更大。 RC和漂样空间内的可行簇似乎与早期后损伤后的新生血管相关有关。少量的ESC移植在RC染色的用于成熟神经元或胶质细胞标记物中。 ESC在几个地点幸存下来漂浮在露棕榈中，但大多数分化为胶质胶质细胞。如果有的话，escs移植到内秋体中幸存下来。这些实验表明，在损伤后期发生的植入和生存中存在最佳时间窗口。

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