...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular biochemistry. >Ablation alleviates atrial fibrillation by regulating the signaling pathways of endothelial nitric oxide synthase/nitric oxide via miR‐155‐5p and miR‐24‐3p
【24h】

Ablation alleviates atrial fibrillation by regulating the signaling pathways of endothelial nitric oxide synthase/nitric oxide via miR‐155‐5p and miR‐24‐3p

机译：通过MiR-155-5P和MIR-24-3P调节内皮一氧化氮合酶/一氧化氮的信号通路来缓解心房颤动

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Background This study aimed to identify the microRNAs implicated in atrial fibrillation (AF) to investigate the molecular mechanisms underlying the role of microRNAs in ablation‐based AF treatment. Methods Real‐time polymerase chain reaction (PCR)?and microRNA microarrays were utilized to measure the profiles of microRNA expression in AF to identify differentially expressed microRNAs. Enzyme‐linked immunosorbent assay, real‐time PCR, Western blot analysis, and immunohistochemistry assays were also performed to investigate the regulatory relationships among various factors implicated in AF. Finally, bioinformatic tools and luciferase assays were used to confirm the roles of miR‐155‐5p, miR‐24‐3p, endothelial nitric oxide synthase (eNOS), and nitric oxide (NO) in the pathogenesis of AF. Results We demonstrated that the levels of miR‐155‐5p and miR‐24‐3p were greatly reduced in postablation AF patients compared with those AF patients not treated by ablation. Furthermore, the NO level in the AF+ group was much lower than that of the AF? group. Finally, in a swine model of AF, evident upregulation of miR‐155‐5p and miR‐24‐3p was found in AF pigs, whereas the ablation treatment reduced the levels of miR‐155‐5p and miR‐24‐3p in AF pigs. On contrary, as targets of miR‐155‐5p and miR‐24‐3p, the levels of eNOS and NO increased when the expression of miR‐155‐5p and miR‐24‐3p decreased. Conclusion MiR‐155‐5p and miR‐24‐3p are involved in the pathogenesis of AF via regulating the expression of eNOS and the production of NO. In addition, ablation treatment helps the recovery from AF by reducing the expression of miR‐155‐5p and miR‐24‐3p.
机译：摘要背景本研究旨在鉴定含有心房颤动（AF）的微小RNA，以研究微大RNA在基于消融的AF处理中的作用的分子机制。方法采用实时聚合酶链式反应（PCR）吗？和MicroRNA微阵列测量AF中微小RNA表达的谱，以识别差异表达的微小RNA。酶联免疫吸附测定，实时PCR，Western印迹分析和免疫组织化学分析也进行了研究，以研究AF中涉及的各种因素之间的调节关系。最后，使用生物信息工具和荧光素酶测定来证实miR-155-5p，miR-24-3p，内皮一氧化物合酶（内皮一氧化氮合酶（eNOS）和一氧化氮（NO）的作用。结果我们证明，与未被消融治疗的AF患者相比，MIR-155-5P和MIR-24-3P的水平大大降低了。此外，AF +组中的没有水平远低于AF的水平？团体。最后，在AF的猪模型中，在AF猪中发现了miR-155-5p和miR-24-3p的明显上调，而消融处理降低了af中的miR-155-5p和miR-24-3p的水平猪。相反，作为miR-155-5p和miR-24-3p的靶标，当miR-155-5p和miR-24-3p的表达减少时，eNOS的水平和不增加。结论MIR-155-5P和MIR-24-3P参与AF通过调节ENOS表达和NO的产生的发病机制。此外，消融处理通过降低miR-155-5p和miR-24-3p的表达，有助于从AF中恢复。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号