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首页> 外文期刊>Journal of cellular biochemistry. >A mitogenic lectin from Rhizoctonia bataticola Rhizoctonia bataticola arrests growth, inhibits metastasis, and induces apoptosis in human colon epithelial cancer cells
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A mitogenic lectin from Rhizoctonia bataticola Rhizoctonia bataticola arrests growth, inhibits metastasis, and induces apoptosis in human colon epithelial cancer cells

机译：来自Rhizoctonia Batatocatola Rhizoctonia Batatocatola的促霉素抑制生长，抑制转移，并在人结肠上皮癌细胞中诱导细胞凋亡

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Abstract The correlation between colorectal cancer (CRC) progression and altered expression of N ‐glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N‐glycan specific mitogenic lectin from Rhizoctonia bataticola (RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here, we report the effect of RBL on human colon cancer HT 29, SW480, and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404, and 192, respectively for HT 29, SW480, and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC 50 of 5, 6.4, and 6.8?μg/mL, respectively for HT 29, SW480, and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1%, 14.3%, and 23.2%, respectively at 12, 24, and 36?h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion, and migration assays demonstrated anti‐metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent.
机译：摘要可以考虑结直肠癌（CRC）进展和改变N-Glycans表达的相关性，以寻找新的生物标志物和抗癌剂来控制CRC。据报道，来自Rhizoctonia Batatocatola（RBL）的N-Glycan特异性促霉菌凝集素，其对人卵巢和白血病细胞具有生长抑制和凋亡作用，但对正常PBMC揭示其临床潜力的致动作用。在此，我们报告RBL对人结肠癌HT 29，SW480和SW620细胞生长及其与人正常结肠癌和癌组织的差异结合的影响。 RBL分别对HT 29，SW480和SW620细胞分别具有403,404和192的MFI的主要和转移性结肠癌细胞的强烈结合。 RBL分别显示用于HT 29，SW480和SW620细胞的IC 50的剂量和时间依赖性生长抑制效果，共5例，6.4和6.8μg/ mL。 RBL抑制结肠癌细胞的克隆因性。 RBL在S相中抑制转移性SW620细胞生长，增加了低倍数群，分别在12,24和36μl中增加了6.1％，14.3％和23.2％。此外，RBL以时间依赖性方式诱导SW620细胞凋亡，与凝集素未处理的对照相比，ROS和核降解的释放增加。附着力，伤口愈合，侵袭和迁移测定证明了SW620细胞中RBL的抗转移效应与其生长抑制作用。通过CAM测定证明RBL的抗血管生成效果。所有这些结果显示RBL的有希望的潜力，既是诊断和治疗剂。

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