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首页> 外文期刊>日本線虫学会誌 >Detection of the root-lesion nematode, Pratylenchus penetrans (Cobb), in a nematode community using real-time PCR
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Detection of the root-lesion nematode, Pratylenchus penetrans (Cobb), in a nematode community using real-time PCR

机译：使用实时PCR检测Nematode群落中的根病变线虫，pratylenchus penetrans（cobb）

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摘要

Pratylenchus penetrans causes damage to different vegetables such as radish, carrot, burdock, lettuce, butterbur, and strawberry (Chikaoka, 1983) and is considered as one of the most important nematode pests for Japanese agriculture. Because it is known that there is a significant correlation between the population density of P. penetrans in soil and degree of damage on the host (Chikaoka, 1983; Ohbayashi, 1989), reliable and rapid identification and counts of the causal agent is critically importantfor successful management of the nematode pest. Mandani et al. (2005) also emphasized that precise identification and knowledge about the density of nematodes in field soil are necessary to develop effective integrated pest control. However, identification of nematodes based on their morphological characteristics is sometimes difficult, especially for inexperienced nematologists. In addition, to detect a single P. penetrans individual in a sample with an abundant number of nematodes is tedious and time-consuming.
机译：Pratylenchus Penetrans对不同的蔬菜造成损坏，如萝卜，胡萝卜，牛蒡，莴苣，乳房和草莓（Chikaoka，1983），并且被认为是日本农业最重要的线虫害虫之一。因为众所周知，在土壤中的P. penetrans的人口密度与主持人的损伤程度之间存在显着相关性（Chikaoka，1983; Ohbayashi，1989），可靠且快速鉴定和因果因计数至关重要成功管理线虫害虫。 Mandani等人。 （2005）还强调，有关现场土壤中线虫密度的精确鉴定和知识是开发有效的综合虫害控制所必需的。然而，基于它们的形态特征鉴定线虫有时是困难的，特别是对于缺乏经验的线虫学家。此外，在具有丰富数量的线虫的样本中检测单个P.穿孔单独的乏味且耗时。

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