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首页> 外文期刊>American journal of primatology >Effectiveness of Saliva Collection and Enzyme-Immunoassay for the Quantification of Cortisol in Socially Housed Baboons
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Effectiveness of Saliva Collection and Enzyme-Immunoassay for the Quantification of Cortisol in Socially Housed Baboons

机译：唾液收集和酶联免疫吸附法测定社交性狒狒中皮质醇的功效

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Circulating cortisol levels are often used to assess the biological stress response in captive primates. Some methods commonly used to collect blood samples may alter the stress response. As such, noninvasive means to analyze cortisol levels are increasingly being developed. We adapted an existing collection method to simultaneously obtain saliva from multiple socially living hamadryas baboons (Papio hamadryas hamadryas) and validated an enzyme-immunoassay kit to quantify cortisol within the saliva samples. Over a period of 12 months, saliva samples were regularly collected from approximately half of the 18-member colony, representing younger monkeys who were more willing to participate. The assay met the four criteria typically used to assess the effectiveness of a new analytical technique: parallelism, precision, accuracy, and sensitivity. Cortisol levels were also proportional to those expected given published plasma levels of cortisol in baboons. Further, salivary cortisol levels increased in individuals following significant stress-related events, such as removal from the group, indicating biological validation. The technique provided a reliable and effective means to assess a physiological indicator of stress in a social group without initiating a stress response owing to handling or sedation, and provided a real-time assessment of cortisol levels and reactivity. Am. J. Primatol. 70:1145-1151, 2008. (C) 2008 Wiley-Liss, Inc.
机译：循环皮质醇水平通常用于评估圈养灵长类动物的生物应激反应。通常用于收集血液样本的某些方法可能会改变压力反应。因此，越来越多地开发用于分析皮质醇水平的非侵入性手段。我们采用了一种现有的采集方法，以便同时从多个社交生活的狒狒狒狒（Papio hamadryas hamadryas）中获得唾液，并验证了一种酶联免疫分析试剂盒来定量唾液样品中的皮质醇。在12个月的时间里，定期从大约18个成员的殖民地中的一半收集唾液样本，代表了更愿意参与的年轻猴子。该测定符合通常用于评估新分析技术有效性的四个标准：并行性，准确性，准确性和敏感性。给定狒狒的血浆皮质醇水平，皮质醇水平也与预期水平成正比。此外，在重大的应激相关事件（例如从组中删除）后，个体唾液皮质醇水平增加，表明生物学验证。该技术提供了一种可靠而有效的方法来评估社会群体中压力的生理指标，而不会由于处理或镇静而引起压力反应，并提供了皮质醇水平和反应性的实时评估。上午。 J. Primatol。 70：1145-1151，2008.（C）2008 Wiley-Liss，Inc.

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