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首页> 外文期刊>Allergy >Identification of spore allergens from the indoor mould Aspergillus versicolor.
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Identification of spore allergens from the indoor mould Aspergillus versicolor.

机译：从室内霉变黑曲霉鉴定孢子变应原。

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BACKGROUND: Indoor mould growth and dampness are associated with respiratory health effects and allergies and several studies demonstrated that mainly Aspergillus versicolor and Penicillium expansum are responsible for indoor mould exposure. In contrast, commercialized test systems to diagnose allergic reactions to this mould species are not available. In this study, allergenic proteins from spores of the indoor relevant species A. versicolor and P. expansum should get detected and identified. METHODS: We used two-dimensional (2D)-gel electrophoresis of spore proteins and immunoblotting with sera from patients participating in an epidemiologic study about indoor exposure of moulds and their influence on the development of allergies (ESTERSPEGA). Sera were screened for IgE antibodies specific for proteins from A. versicolor, A. fumigatus and P. expansum in one-dimensional blots and in 2D immunoblots. From the 2D gels, the corresponding spots were picked and identified by mass spectrometry. RESULTS: Morethan 20 allergens from A. versicolor were identified; in particular, seven major allergens were selected, which were detected by more than 90% of the positive sera. The most abundant allergen was glyceraldehyde-3-phosphate dehydrogenase, followed by an unnamed protein, which displays a high homology to sobitol/xylose reductase. The other allergens were identified as catalase A, hypothetical protein AN6918.2, enolase, hypothetical protein AN0297.2 and a protein with homology to a fungal malate dehydrogenase. CONCLUSIONS: The results indicate an important role of spore proteins from A. versicolor for sensitization against indoor moulds and identification of the major allergens might enable species-specific diagnosis of allergic reactions.
机译：背景：室内霉菌的生长和潮湿与呼吸健康和过敏有关，多项研究表明，室内霉菌的暴露主要是杂色曲霉和扩张青霉。相反，没有可用于诊断对该霉菌种过敏反应的商业化测试系统。在这项研究中，应该检测和鉴定来自室内相关物种杂色曲霉和膨胀假单胞菌的孢子的致敏蛋白。方法：我们使用了孢子蛋白的二维（2D）凝胶电泳，并对参与霉菌的室内流行病学研究的患者进行了血清血清免疫印迹（ESTERSPEGA）。在一维印迹和二维免疫印迹中，针对血清中特异于杂色曲霉，烟曲霉和膨胀假单胞菌的蛋白质筛选IgE抗体。从2D凝胶中，挑出相应的斑点并通过质谱鉴定。结果：从杂色曲霉中鉴定出超过20种过敏原。特别是，选择了七种主要的过敏原，它们被超过90％的阳性血清检测到。最丰富的过敏原是3-磷酸甘油醛脱氢酶，其次是一个未命名的蛋白，该蛋白与sobitol /木糖还原酶具有高度同源性。其他过敏原被鉴定为过氧化氢酶A，假定蛋白AN6918.2，烯醇酶，假定蛋白AN0297.2和与真菌苹果酸脱氢酶同源的蛋白。结论：结果表明杂色曲霉孢子蛋白对室内霉菌的致敏作用很重要，主要过敏原的鉴定可能有助于对变态反应进行物种特异性诊断。

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