...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Allergy >Alterations of the ocular surface epithelial MUC16 and goblet cell MUC5AC in patients with atopic keratoconjunctivitis.
【24h】

Alterations of the ocular surface epithelial MUC16 and goblet cell MUC5AC in patients with atopic keratoconjunctivitis.

机译：特应性角结膜炎患者眼表上皮MUC16和杯状细胞MUC5AC的变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: An increased understanding of the ocular surface at cellular level in the conjunctiva and the cornea may help explain the pathogenesis and the subsequent clinical appearance of atopic ocular allergies, which may be potentially blinding. PURPOSE: To investigate the MUC16 and MUC5AC alterations, tear function and the ocular surface disorder in patients with atopic keratoconjunctivitis (AKC). METHODS: Thirty-six eyes of 18 AKC patients as well as 28 eyes of 14 age- and sex-matched normal subjects were studied. The subjects underwent corneal sensitivity measurements, Schirmer test, tear film break-up time (BUT), fluorescein and Rose-Bengal staining of the ocular surface, conjunctival impression cytology and brush cytology. Impression cytology samples underwent periodic acid schiff and immunohistochemical staining with MUC16 and MUC5AC antibodies. Brush cytology specimens underwent evaluation for inflammatory cell numbers and quantitative real-time polymerase chain reaction (PCR) for MUC16 and MUC5AC mRNA expression. RESULTS: The mean corneal sensitivity and BUT values were significantly lower in patients with AKC, compared with controls (P < 0.001). Brush cytology specimens from AKC patients revealed significantly higher numbers of inflammatory cells (P < 0.001). Specimens from patient eyes showed positive staining for MUC5AC and MUC16. MUC16 mRNA expression was significantly upregulated with significant downregulation of MUC5AC mRNA expression in eyes with AKC compared with the eyes of control subjects. CONCLUSION: Ocular surface inflammation, decline in corneal sensitivity, tear film instability, changes in conjunctival epithelial MUC5AC and MUC16 mRNA expressions were thought to be important in the pathogenesis of atopic ocular surface disease.
机译：背景：对结膜和角膜细胞水平的眼表了解的增加，可能有助于解释特应性眼过敏的发病机理和随后的临床表现，这可能会致盲。目的：探讨特应性角结膜炎（AKC）患者的MUC16和MUC5AC改变，泪液功能和眼表疾病。方法：对18例AKC患者的36眼以及14例年龄和性别匹配的正常受试者的28眼进行了研究。受试者进行了角膜敏感性测量，Schirmer测试，泪膜破裂时间（BUT），荧光素和眼表的Rose-Bengal染色，结膜印象细胞学和刷细胞学。印象细胞学样品用MUC16和MUC5AC抗体进行高碘酸希夫（Schiff）和免疫组化染色。刷细胞学标本进行了评估炎症细胞数量和定量实时聚合酶链反应（PCR）的MUC16和MUC5AC mRNA表达。结果：与对照组相比，AKC患者的平均角膜敏感性和BUT值显着降低（P <0.001）。 AKC患者的刷细胞学标本显示炎症细胞数量明显更高（P <0.001）。来自患者眼睛的标本显示MUC5AC和MUC16呈阳性染色。与对照组相比，AKC眼中MUC16 mRNA表达显着上调，而MUC5AC mRNA表达则显着下调。结论：眼表炎症，角膜敏感性下降，泪膜不稳定性，结膜上皮MUC5AC和MUC16 mRNA表达的变化被认为在特应性眼表疾病的发病机制中起重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号