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首页> 外文期刊>Allergy >Basophil allergen threshold sensitivity: a useful approach to anti-IgE treatment efficacy evaluation.
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Basophil allergen threshold sensitivity: a useful approach to anti-IgE treatment efficacy evaluation.

机译：嗜碱性粒细胞变应原阈值敏感性：一种抗IgE治疗功效评估的有用方法。

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Background: Monitoring of the allergen sensitivity of a patient is most important for optimal patient care and a basic prerequisite for immunomodulating treatment. The objective of this study was to investigate how basophil allergen sensitivity can be applied in the monitoring of anti-immunoglobulin E (IgE) treatment. Methods: Basophils from timothy grass pollen allergic patients were, by flow cytometry, analysed for allergen threshold sensitivity (CD-sens) by measuring CD63 up-regulation on CD203c-identified basophils. The results were compared with maximal percentage CD63 up-regulation at one allergen dose (CD-max), skin prick test end-point allergen titration, (SPT-sens), nasal provocation titration tests (nasal provocation titre) and serum IgE and IgE antibody concentrations. Results: There was a significant correlation (r = 0.50, P = 0.01) between CD-sens and SPT-sens, CD-sens and the IgE antibody concentration in percentage of 'total IgE' (relative IgE antibody concentration) (r = 0.72, P < 0.001) as well as between CD-sens and nasal provocation titre (r = 0.54, P < 0.05) but, in contrast, CD-max did not correlate with any of the sensitization parameters, i.e. SPT-sens, nasal provocation titre, absolute and relative IgE antibody concentration or CD-sens. CD-sens could be used to monitor omalizumab treatment efficacy while, based on CD-max, four of seven symptom-free patients on omalizumab would have been classified as having ongoing allergy. Conclusions: CD-sens seems to be very useful for the determination of a patient's allergen sensitivity and should be evaluated for the measurement and monitoring of anti-IgE treatment efficacy. CD-max, the conventional approach to basophil allergen challenge, which mirrors cell reactivity, gives incorrect information.
机译：背景：对患者过敏原敏感性的监测对于最佳患者护理和免疫调节治疗的基本前提至关重要。这项研究的目的是研究如何将嗜碱性粒细胞变应原敏感性应用于监测抗免疫球蛋白E（IgE）的治疗。方法：采用流式细胞术，对来自提摩太花粉花粉过敏患者的嗜碱性粒细胞，通过测量CD203c鉴定的嗜碱性粒细胞上CD63的上调来分析过敏原阈值敏感性（CD-sens）。将结果与一种过敏原剂量下最大CD63上调百分比（CD-max），皮肤点刺试验终点过敏原滴定法（SPT-sens），鼻腔激发滴定试验（鼻腔激发滴度）以及血清IgE和IgE进行比较抗体浓度。结果：CD-sens和SPT-sens，CD-sens与IgE抗体浓度之间的显着相关性（r = 0.50，P = 0.01），占“总IgE”（相对IgE抗体浓度）的百分比（r = 0.72） ，P <0.001）以及CD感官与鼻挑衅滴度之间的相关性（r = 0.54，P <0.05），但相比之下，CD-max与任何敏化参数（SPT感官，鼻挑衅）均不相关。滴定度，绝对和相对IgE抗体浓度或CD-sens。 CD-sens可用于监测奥马珠单抗的治疗效果，而基于CD-max，奥马珠单抗的7名无症状患者中有4名将被分类为持续过敏。结论：CD-sens似乎对于确定患者的过敏原敏感性非常有用，应评估其抗IgE治疗效果的测量和监测。 CD-max是嗜碱性粒细胞变应原攻击的常规方法，反映了细胞反应性，提供了错误的信息。

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