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首页> 外文期刊>Allergy >Exposure to cadmium-contaminated soils increases allergenicity of Poa annua L. pollen.
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Exposure to cadmium-contaminated soils increases allergenicity of Poa annua L. pollen.

机译：暴露在镉污染的土壤中会增加Poa annua L.花粉的致敏性。

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BACKGROUND: Pollution is considered as one main cause for the increase of allergic diseases. Air pollutants may cause and worsen airway diseases and are probably able to make pollen allergens more aggressive. Previous studies looked at traffic-related air pollution, but no data about the effects of polluted soils on pollen allergens are available. We aimed to assess the effects of plant exposure to cadmium-contaminated soil on allergenicity of the annual blue grass, Poa annua L, pollen. METHODS: Poa plants were grown in soil contaminated or not contaminated (control) with cadmium. At flowering, mature pollen was analyzed by microscopy, to calculate the percentage of pollen grains releasing cytoplasmic granules, and by proteomic techniques to analyze allergen proteins. Allergens were identified by sera from grass pollen-allergic patients and by mass spectrometry. RESULTS: Pollen from Cd-exposed plants released a higher amount of allergenic proteins than control plants. Moreover, Cd-exposed pollen released allergens-containing cytoplasmic grains much more promptly than control pollen. Group 1 and 5 allergens, the major grass pollen allergens, were detected both in control and Cd-exposed extracts. These were the only allergens reacting with patient's sera in control pollen, whereas additional proteins strengthening the signal in the gel region reacting with patient's sera were present in Cd-exposed pollen. These included a pectinesterase, a lipase, a nuclease, and a secretory peroxydase. Moreover, a PR3 class I chitinase-like protein was also immunodetected in exposed plants. CONCLUSION: Pollen content of plants grown in Cd-contaminated soils is more easily released in the environment and also shows an increased propensity to bind specific IgE.
机译：背景：污染被认为是过敏性疾病增加的主要原因之一。空气污染物可能导致并加剧呼吸道疾病，并可能使花粉过敏原更具侵略性。先前的研究着眼于交通相关的空气污染，但尚无有关污染土壤对花粉过敏原影响的数据。我们的目的是评估植物暴露于镉污染的土壤对一年生青草Poa annua L花粉的致敏性的影响。方法：Poa植物生长在受镉污染或未受镉污染（对照）的土壤中。在开花时，通过显微镜分析成熟的花粉，以计算释放细胞质颗粒的花粉粒的百分比，并通过蛋白质组学技术分析变应原蛋白。通过草花粉过敏患者的血清和质谱鉴定过敏原。结果：暴露于Cd的植物中的花粉释放的过敏原蛋白量高于对照植物。而且，暴露于镉的花粉比对照花粉更迅速地释放出含过敏原的细胞质颗粒。在对照组和暴露于Cd的提取物中均检测到第1组和第5组过敏原，这是主要的草花粉过敏原。这些是在对照花粉中与患者血清反应的唯一变应原，而在暴露于Cd的花粉中存在增强与患者血清反应的凝胶区域中信号的其他蛋白质。这些包括果胶酯酶，脂肪酶，核酸酶和分泌性过氧化物酶。此外，在暴露的植物中也免疫检测到PR3 I类几丁质酶样蛋白。结论：在受Cd污染的土壤中生长的植物的花粉含量更容易在环境中释放，并且结合特定IgE的倾向也增加。

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