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首页> 外文期刊>Allergy >Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps.
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Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps.

机译：鼻息肉中基质金属蛋白酶MMP-2，MMP-9，基质金属蛋白酶-1的组织抑制剂与IL-5，IL-8之间的关系。

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BACKGROUND: Nasal polyps (NP), a subgroup of chronic rhinosinusitis, are characterized by interleukin 5 (IL-5) mediated infiltration of eosinophils in sinus mucosa, leading to pseudostratified ciliated columnar epithelium, thickening of the epithelial basement membrane and tissue edema. Matrix metalloproteinases (MMP) constitute a large group of Zn2+ dependent endopeptidases with the ability to degrade extracellular matrix and are possibly responsible for the development of tissue edema in chronic sinusitis. OBJECTIVE: The aim of this study was to determine the expression of MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) mRNA and to locate the distribution of MMP-2, MMP-9 and TIMP-1 by immunohistochemistry in ethmoid sinus mucosa in NP. Furthermore the correlation between IL-5 or IL-8 and MMP-2, MMP-9 or TIMP-1 is examined. METHODS: Nasal polyps of 33 patients and 18 specimens of inferior turbinate mucosa were examined by real time RT-PCR for MMP-2, MMP-9, TIMP-1, IL-5 and IL-8 mRNA expression. Immunohistochemical labeling for MMP-2, MMP-9 and TIMP-1 was performed. RESULTS: Differences between both locations were detectable for MMP-9 (P < 0.001) and IL-5 (P=0.003) but not for MMP-2 (P=0.278), TIMP-1 (P=0.515) and IL-8 (P=0.386). Correlation was detected only between TIMP-1 and IL-5 (r=0.422, P =0.014). Cytoplasmic staining of MMP-2 was present in the apical part of the ciliated cells, submucosal glands and in smooth muscle cells. Matrix metalloproteinase-9 was expressed in surface epithelium, in seromucous glands and in polymorphonuclear cells. CONCLUSIONS: Expression of MMP-9 and IL-5 mRNA are associated with NP. The correlation between IL-5 and TIMP-1 indicates the role of TIMP-1 in maintaining the homeostasis in NP.
机译：背景：鼻息肉（NP）是慢性鼻-鼻窦炎的一个亚组，其特征是白介素5（IL-5）介导的嗜酸性粒细胞浸润在窦粘膜中，导致假复层纤毛状柱状上皮，上皮基底膜增厚和组织水肿。基质金属蛋白酶（MMP）构成了一大部分Zn2 +依赖的内肽酶，具有降解细胞外基质的能力，可能与慢性鼻窦炎组织水肿的发展有关。目的：研究MMP-2，MMP-9和基质金属蛋白酶-1（TIMP-1）mRNA的组织抑制剂的表达，并确定MMP-2，MMP-9和TIMP-筛查窦房结黏膜中NP的免疫组化结果为1。此外，检查了IL-5或IL-8与MMP-2，MMP-9或TIMP-1之间的相关性。方法：实时RT-PCR检测33例鼻息肉和18例下鼻甲黏膜标本中MMP-2，MMP-9，TIMP-1，IL-5和IL-8 mRNA的表达。进行了MMP-2，MMP-9和TIMP-1的免疫组织化学标记。结果：MMP-9（P <0.001）和IL-5（P = 0.003）的两个位置之间的差异是可检测到的，而MMP-2（P = 0.278），TIMP-1（P = 0.515）和IL-8的两个位置之间没有差异。 （P = 0.386）。仅在TIMP-1和IL-5之间检测到相关性（r = 0.422，P = 0.014）。 MMP-2的细胞质染色存在于纤毛细胞的顶端，粘膜下腺和平滑肌细胞中。基质金属蛋白酶9在表面上皮，浆液腺和多形核细胞中表达。结论：MMP-9和IL-5 mRNA的表达与NP有关。 IL-5与TIMP-1之间的相关性表明TIMP-1在维持NP稳态中的作用。

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