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首页> 外文期刊>Allergy >Extracellular DNA traps in bronchoalveolar fluid from a murine eosinophilic pulmonary response
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Extracellular DNA traps in bronchoalveolar fluid from a murine eosinophilic pulmonary response

机译：鼠嗜酸性粒细胞肺反应中支气管肺泡液中的细胞外DNA陷阱

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摘要

Asthma is associated with a loss of the structural integrity of airway epithelium and dysfunction of the physical barrier, which protects airways from external harmful factors. Granulocyte activation causes the formation of extracellular traps, releasing web-like structures of DNA and proteins, being important to kill pathogens extracellularly. We investigated whether eosinophils infiltrating airways in an experimental model of asthma would induce eosinophil extracellular traps (EETs) in bronchoalveolar lavage fluid and lung tissue. We showed that an ovalbumin (OVA) asthma protocol presented a significant increase in eosinophil counts with increased extracellular DNA in bronchoalveolar lavage fluid as well as in lung tissue, confirming the presence of DNA traps colocalized with eosinophil peroxidase. EETs formation was reversed by DNase treatment. With these approaches, we demonstrated for the first time that OVA-challenged mice release extracellular DNA traps, which could aggravate pulmonary dysfunction.
机译：哮喘与气道上皮结构完整性的丧失和物理屏障的功能障碍有关，从而保护了气道免受外界有害因素的侵害。粒细胞活化导致细胞外陷阱的形成，释放出DNA和蛋白质的网状结构，这对于在细胞外杀死病原体很重要。我们调查了哮喘实验模型中浸润气道的嗜酸性粒细胞是否会在支气管肺泡灌洗液和肺组织中诱导嗜酸性粒细胞胞外陷阱（EET）。我们显示，卵白蛋白（OVA）哮喘方案在支气管肺泡灌洗液以及肺组织中的嗜酸性粒细胞计数显着增加，而胞外DNA则增加，这证实了与嗜酸性粒细胞过氧化物酶共定位的DNA陷阱的存在。 DNase处理可逆转EET的形成。通过这些方法，我们首次证明了由OVA攻击的小鼠释放出细胞外DNA陷阱，这会加剧肺功能障碍。

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