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Mutation of penicillin-binding protein genes and antimicrobial susceptibility of Haemophilus influenzae isolated from spinal fluid or blood in children

机译:青霉素结合蛋白基因的突变与儿童脊髓液中嗜血杆菌嗜血杆菌的抗微生物敏感性

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摘要

Between September 1999 and August 2001, we studied serotypes to capsular antigen, beta-lactamase production, mutation of penicillin binding protein (PBP) genes by PCR method, and antimicrobial susceptibilities of 13 strains of Haemophilus influenzae isolated from spinal fluid or blood in children. Diseases of patients were meningitis in 11, pneumonia in 1, and laryngitis in 1. The age range of the patients was from 26 days to 5 years. The serotypes of all strains were b. Four of the 13 strains were beta-lactamase-positive. The mutation of genes of pbp3 was revealed from 4 isolates and 2 of the strains were beta-lactamase-positive. MICs of ampicillin to beta-lactamase-negative strains ranged from 0.125 to 1 microgram/ml and those to beta-lactamase-positive were more than 32 micrograms/ml. MICs of 2 strains of beta-lactamase-negative and mutation-positive were 0.5 and 1 microgram/ml. The excellent active antimicrobials in our study was cefotaxime (MIC90 0.06 microgram/ml), meropenem (MIC90 0.125 microgram/ml), ceftazidime (MIC90 0.25 microgram/ml), and cefepime (MIC90 0.25 microgram/ml).
机译:1999年9月至2001年8月期间,我们通过PCR方法研究了血清抗原,β-内酰胺酶生产,青霉素结合蛋白(PBP)基因的突变,以及13株嗜血杆菌嗜血杆菌的抗微生物敏感性。患者的疾病是11,肺炎1,肺炎和喉炎。患者的年龄范围为26天至5年。所有菌株的血清型都是b。 13个菌株中的四种是β-内酰胺酶阳性。 PBP3基因的突变从4个分离物中揭示,其中2个菌株是β-乳酰胺酶阳性。氨苄青霉素对β-内酰胺酶阴性菌株的麦克风范围为0.125至1微克/ ml,β-乳酰胺酶阳性的含量为32微克/ ml。 β-内酰胺酶阴性和突变阳性的2个菌株的MIC为0.5和1微克/ mL。我们研究中优异的活性抗微生物剂是头孢噻肟(MIC90 0.06微克/ mL),MEROPENEM(MIC90 0.125微克/ mL),头孢他啶(MIC90 0.25微克/ mL),以及头孢噻肟(MIC90 0.25微克/ mL)。

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