• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>感染症学雑誌 >Analysis of an antigen in Chlamydia trachomatis infected-cell extract which reacts with sera of patients with C. trachomatis infection
【24h】

Analysis of an antigen in Chlamydia trachomatis infected-cell extract which reacts with sera of patients with C. trachomatis infection

机译：衣原体抗原感染细胞提取物的分析与C. Thachomatis感染患者血清反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To find a new marker for sero-diagnosis of Chlamydia trachomatis infection, Western blot (WB) method was performed by using C. trachomatis infected HeLa229 cell extract (L2-ext) as antigen. Two series of sera of patients with pneumonia or cervicitis (4 sera each) were used for investigation and analysis of a band which fades earlier than others depending on weeks or antibody levels after therapeutic treatment. A 17 KDa band was found which tended to fade gradually, but did not completely disappear within the period of investigation. The band was also detected in sera of patients with cervicitis diagnosed by detection of C. trachomatis organisms (IDEIA-PCE Chlamydia (IDEIA) or Clearview test were positive at first visit to the clinic). Ten anti-C. pneumoniae antibody positive sera and five anti-Mycoplasma pneumoniae antibody positive sera were also tested as controls. The result was that a 17 KDa band was detected in 20 of 25 sera (80%) with IDEIA positive. 18 of 33 patients (54.5%) with Clearview positive-, and 12 of 16 (75%) sera with both tests positive. No band was found in the control sera. The frequency of antibody against 17 KDa antigen was almost completely identical with that obtained by microimmunofluorescence test and Peptide-Chlamydia-IgG test. These results show that a 17 KDa band may be used as a marker for detection of C. trachomatis antibody by the WB method. The antigen could be precipitated with salting out from the L2-ext with 60% saturation of ammonium sulfate of Hofmeister's method and it was digested with proteinase K. From the result of the amino acid sequence analysis, it was found that 17 KDa protein is the human nucleoside diphosphate kinase B.
机译：为了找到沙眼衣原体感染的血清诊断新的标记，通过使用沙眼衣原体感染HeLa229细胞提取物（L2-EXT）作为抗原进行Western印迹（WB）方法。两个系列的肺炎患者或宫颈炎（各4血清）血清被用于一个波段的调查和分析其衰落比别人更早取决于周或治疗后抗体水平。一名17 kDa带发现它倾向于将逐渐淡化，但调查期间内并没有完全消失。在患者的宫颈炎通过检测C的诊断血清也检测的频带衣原体生物体（IDEIA-PCE衣原体（IDEIA）或Clearview的测试呈阳性反应在第一次访问到诊所）。十防-C。肺炎抗体阳性血清和5抗肺炎支原体抗体阳性血清也进行了测试作为对照。其结果是，在25血清（80％）与IDEIA阳性的20检测到17kDa的条带。的33名患者（54.5％）与Clearview的正性，并与这两个测试阳性16（75％）的血清的12 18。没有乐队在对照血清中发现。抗体对17kDa的抗原的频率是与通过微量免疫荧光试验和肽衣原体-IgG的试验得到几乎完全一致。这些结果表明，17 kDa带也可以用作用于检测沙眼衣原体抗体的由WB方法的标记。所述抗原可以与从L2-EXT与硫酸霍夫迈斯特的方法的铵的60％饱和度的盐析沉淀并用蛋白酶K消化，从氨基酸序列分析的结果，人们发现，17 kDa蛋白是人类核苷二磷酸激酶B.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号