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首页> 外文期刊>感染症学雑誌 >Studies on Vibrio vulnificus infection: molecular epidemiology of environment-derived strains and clinical isolates
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Studies on Vibrio vulnificus infection: molecular epidemiology of environment-derived strains and clinical isolates

机译：vibrioulnfificus感染研究：环境衍生菌株的分子流行病学和临床分离株

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To clarify the route and source of Vibrio vulnificus infection, we conducted molecular epidemiological investigation by DNA analysis of 355 environmental isolates (seawater-derived strain: 86, sea mud-derived strain:36, and oyster-derived strain: 233) and 65 human clinical isolates, for a total of 420 isolates, using pulse field gel electrophoresis (PFGE), with the following results. 1. When DNA was cleaved with 2 enzymes, Not I and Sfi I, and subjected to PFGE, Not I DNA interpretation was 76.9%, and Sfi I cleavage was 97.9%, showing that Sfi I was superior in cleaving DNA of this bacteria. 2. Sfi I-interpreted strains were subjected to PFGE and migration patterns were analyzed by UPGMA, but close classification was not possible because similarity was low, this infectious disease clearly originated from multiple rather than a single-clone. In this cluster, we concluded that this infectious disease was acquired through contact between the environment and human beings and viceversa. We identified an assortment of clinical isolates and environment-derived strains among more than 89% of strain groups tested, none of which could be expected to have the same origin. We conclued DNA analysis on these two types of restriction enzymes using PFGE, but were unable to classify test results in detail due to the proliferation of migration patterns and low degree of similarity.
机译：为了澄清vibrio wulnificus感染的途径和来源，我们通过355环境分离株的DNA分析进行了分子流行病学调查（海水衍生菌株：86，海泥衍生菌株：36和Oyster衍生的菌株：233）和65人临床分离物，总共420个分离物，使用脉冲场凝胶电泳（PFGE），具有以下结果。 1.当DNA用2个酶切割时，不是I和SFI I，并对PFGE进行PFGE，而不是I DNA解释为76.9％，SFI I裂解为97.9％，表明SFI在这种细菌的裂解DNA方面是优越的。 2. SFI I-解释的菌株对PFGE进行PFGE，通过UPGMA分析迁移模式，但不可能进行密切的分类，因为相似性低，这种传染病明显起源于多个而不是单克隆。在该群体中，我们得出结论，通过环境和人类与伏牛皮之间的联系获得了这种传染病。我们鉴定了在测试的超过89％的菌株组中均有各种临床分离物和环境衍生的菌株，其中任何一个都可以预期具有相同的原点。我们在使用PFGGE上的这两种限制酶的DNA分析，但由于迁移模式的增殖和低的相似性，不能对测试结果进行分类。

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