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首页> 外文期刊>感染症学雑誌 >The usefulness of human lung embryonal fibroblast cells (MRC-5) for isolation of enteroviruses and adenoviruses
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The usefulness of human lung embryonal fibroblast cells (MRC-5) for isolation of enteroviruses and adenoviruses

机译：人肺胚胎成纤维细胞（MRC-5）用于分离肠病毒和腺病毒的有用性

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Although various cell lines have been used for virus isolation, few study of virus isolation using MRC-5 cell, a human embryonic lung fibroblasts, have been reported in Japan. MRC-5 and other cell lines (Caco-2, Vero, RD-18s, LLC-MK2, HeLa, MDCK, FL, B95a and HMV-II), and suckling mouse were compared for isolation of viruses from clinical specimens. A total of 3,284 specimens, collected from clinics and hospitals in Saitama Prefecture between July 1997 and August 2001, were inoculated in these cells. A total of 1,252 viral strains were isolated and 1,190 viral strains of these were identified. MRC-5 detected 209 of specimens positive for various viruses. As for adenovirus, a total of 132 viral strains were isolated using cell lines described above, and 100 of 132 viral strains were isolated in MRC-5. MRC-5 showed the highest sensitivity for isolation of adenovirus 3 and 7 (79.1% and 100%) of all other cells. The sensitivity in isolation of these viruses in HeLa was 58.1% and 50.0%, respectively. It showed that MRC-5 is able to isolate enterovirus, especially coxsackie virus A16 and enterovirus 71 with a high sensitivity (85.7% and 73.7%). RD-18s detected 35.7% and 26.3% of coxsackie virus A16 and enterovirus 71 isolates, LLC-MK2 detected 60.7% and 47.4%, and Vero detected 48.6% and 52.6%, respectively. Coxsackie virus B group was not isolated, except for a few coxsackie virus B 5 strains. Enteroviruses except coxsackie virus A16 and enterovirus 71 were isolated more frequently in Caco-2 and RD-18s. Seven hundred thirteen strains of influenza viruses were isolated in MDCK and Caco-2, but none was isolated in MRC-5. It was probably due to the maintenance medium without trypsin. The isolation rate of herpes simplex virus in Vero was 88.9% and MRC-5 showed 77.8%, it was high secondary to Vero by MRC-5. However, the CPE was detected in a few days in MRC-5, it was earlier than in Vero. The MRC-5 is possible to be maintained without changing the maintenance medium and passaged for 2 weeks, and clear CPE was observed. On the other hand, the disadvantages in using the MRC-5 were that the passage was limited and that the split ratio was only 1:2. However, the MRC-5 was used successfully for virus isolation, especially coxsackie virus A16, enterovirus 71 and adenoviruses, from clinical specimens.
机译：虽然各种细胞系已被用于病毒分离，但是在日本据报道，少数使用MRC-5细胞，人胚胎肺成纤维细胞的病毒分离研究。比较MRC-5和其他细胞系（CaCO-2，Vero，RD-18S，LLC-MK2，HELA，MDCK，FL，B95A和HMV-II），并与临床标本分离病毒。在1997年7月至2001年7月至8月在埼玉县和2001年至8月中收集了3,284个标本，在这些细胞中接种。共分离了1,252种病毒菌株，鉴定了1,190个病毒菌株。 MRC-5检测到各种病毒的209个标本阳性。对于腺病毒，使用上述细胞系分离了总共132条病毒菌株，并且在MRC-5中分离了100种病毒菌株。 MRC-5显示出腺病毒3和7（79.1％和100％）的最高敏感性。在Hela中分离这些病毒的敏感性分别为58.1％和50.0％。结果表明，MRC-5能够将肠道病毒，特别是Coxsackie病毒A16和肠道病毒71分离出高灵敏度（85.7％和73.7％）。 RD-18S检测到35.7％和26.3％的Coxsackie病毒A16和肠道病毒71分离物，LLC-MK2检测到60.7％和47.4％，分别检测到48.6％和52.6％。除了几个Coxsackie病毒B 5菌株外，没有分离Coxsackie病毒B组。除Coxsackie病毒A16和肠道病毒71之外的肠病毒更频繁地在Caco-2和RD-18s中均更繁多。在MDCK和Caco-2中分离了七十三株流感病毒，但在MRC-5中没有分离出来。它可能是由于没有胰蛋白酶的维护培养基。 Vero中单纯疱疹病毒的分离率为88.9％，MRC-5显示77.8％，MRC-5高次级到Vero。然而，在MRC-5的几天内检测到CPE，它早于VERO。可以在不改变维护介质并传代2周的情况下保持MRC-5，并且观察到清晰的CPE。另一方面，使用MRC-5的缺点是该通道是有限的，并且分裂比仅为1：2。然而，MRC-5成功用于来自临床标本的病毒分离，特别是Coxsackie病毒A16，Entovirus 71和腺病毒。

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