...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>感染症学雑誌 >Comparative study of PCR-based Cryptosporidium discriminating techniques with a review of the literature
【24h】

Comparative study of PCR-based Cryptosporidium discriminating techniques with a review of the literature

机译：基于PCR的隐孢子虫辨别技术对文献综述的对比研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We identified the species or genotypes of the six Cryptosporidium isolates from patients and C. parvum strain HNJ-1 using the seven previously described species-differentiation and genotyping PCR protocols for detection of Cryptosporidium parasites. In addition, we also discussed about the usefulness of these PCR-based protocols on the basis of the reports previously published. Cryptosporidium diagnostic fragment was amplified by PCR with each primer pair, targeting the 18S ribosomal RNA (18SrRNA), Cryptosporidium oocyst wall protein (COWP). Heat shock protein 70 (HSP70), Polythreonine (Poly-T), Thrombospondin related adhesive protein of Cryptosporidium-1 (TRAP-C1), and unknown gene locus, in all isolates from patients and the strain HNJ-1. The RFLP profiles of 18SrRNA, COWP, HSP70, Poly-T, and TRAP-C1 PCR products in all isolates from patients were found to be the same among isolates, and were correspondent to those of C. parvum human genotype. While the RFLP profiles of HNJ-1 were strictly different from those of isolates from patients, and were correspondent to C. parvum cattle genotype. In addition, nucleotide sequences in 18 SrRNA gene of all isolates from patients and HNJ-1 were found to be identical to that of C. parvum, human or cattle genotype, respectively. Therefore, the isolates from patients and HNJ-1 were identified as C. parvum human and cattle genotype, respectively. According to the reports related to the PCR-based protocols applied in the present study, RFLP profiles targeting the HSP70, Poly-T, TRAP-C1 genes had been revealed in only a few species or genotypes, but those of 18SrRNA and COWP genes were in all species and genotypes. However, we supposed that it was difficult to distinguish between human or cattle genotype and other species or genotypes by RFLP profiles of 18SrRNA or COWP because the RFLP profiles of human or cattle genotype were identical or similar to those of other species or genotypes. On the other hand, it has been known that the nucleotide sequences in 18SrRNA or COWP gene are different among Cryptosporidium species and/or genotypes. Therefore, the direct sequencing method targeting the variable regions which can be used to distinguish among Cryptosporidium species, as well as the genotypes within C. parvum in either 18SrRNA or COWP gene is the most useful tool for accurate identification of Cryptosporidium isolates.
机译：我们使用七个先前描述的物种分化和基因分型PCR方案鉴定了来自患者和C.Prvum菌株HNJ-1的六个水孢菌素分离株的物种或基因型。此外，我们还根据先前已发布的报告讨论了基于PCR的协议的有用性。通过PCR用每个引物对扩增诊断片段，靶向18S核糖体RNA（18SrRNA），密码孢子率壁蛋白（COWP）。在患者和患者的所有分离物中，热休克蛋白70（HSP70），聚酮（Poly-T），隐孢子蛋白-1（Trap-C1）和未知基因座的血栓样蛋白相关粘合剂蛋白质和未知的基因座。在患者的所有分离物中，18SrRNA，COWP，HSP70，Poly-T和TRAP-C1 PCR产物的RFLP型材在分离物中存在相同，并且与C. parvum人类基因型的含量相同。虽然HNJ-1的RFLP概况严格与来自患者的分离物的曲线不同，而且对应于C. parvum牛基因型。此外，发现来自患者和HNJ-1的所有分离株的18个SrRNA基因中的核苷酸序列分别与C.Parvum，人或牛基因型的所有分离物相同。因此，患者和HNJ-1的分离物分别被鉴定为C. parvum人和牛基因型。根据本研究中的基于PCR的基于PCR的方案的报告，靶向HSP70，Poly-T，Trap-C1基因的RFLP谱只揭示了几种或基因型，但18SrRNA和牛仔基因的靶向在所有物种和基因型中。然而，我们认为，由于18SrNA或COWP的RFLP谱，难以区分人或牛基因型和其他物种或基因型，因为人或牛基因型的RFLP谱与其他物种或基因型的RFLP曲线相同或类似。另一方面，已知18SrRNA或牛仔基因中的核苷酸序列在隐孢子虫物种和/或基因型中不同。因此，靶向可用于区分隐孢子虫物质的可变区的直接测序方法以及18SRRNA或牛仔基因的C.Parvum内的基因型是最有用的准确鉴定隐孢子虫分离株的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号