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Quantitative detection of Vibrio vulnificus in seafood

机译：海鲜vibrio pulnfificus的定量检测

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To quantify the number of Vibrio vulnificus in shellfish, we compared the most probable number (MPN) combined with a culture (MPN-culture) or polymerase-chain reaction (PCR) assay (MPN-PCR) to a quantitative PCR assay. Enrichment in alkaline peptone water by MPN was conducted at 25 and 35 degrees C. Enrichment at 35 degrees C was superior or similar to enrichment at 25 degrees C in over 65% of samples by MPNculture and in more than 75% of samples by MPN-PCR assay. V. vulnificus was more easily isolated on chromogenic agar medium during culture, MPN-PCR assay was superior or similar to MPNculture in over 90% of samples by enrichment at 25 degrees C and to over 88% of samples by enrichment at 35 degrees C. The number of V. vulnificus by quantitative PCR assay was similar to that of MPN-PCR assay in 6 of 8 samples but not from MPNculture. V. vulnificus contamination was frequently detected in samples from Kyushu Island.
机译：为了量化贝类中的脉冲裂纹的数量，我们将最可能的数量（MPN）与培养物（MPN-培养物）或聚合酶链反应（PCR）测定（MPN-PCR）与定量PCR测定相结合。 通过MPN在碱性蛋白胨水中的富集在25和35℃下进行。35℃的富集优异或类似于通过MPN培养的65％的样品中的25摄氏度和超过75％的样品中的富集 PCR测定。 V.Vulnificus在培养过程中更容易被隔离在发色琼脂培养基上，MPN-PCR测定通过富集在25摄氏度和35℃下通过富集以超过90％的样品中的90％的样品中的MPN培养物。 通过定量PCR测定的V.Vulnificus的数量类似于8个样品中的6个样品中的MPN-PCR测定的数量，而不是来自MPNCulture的类似MPN-PCR测定。 V. vulnificus污染经常在九州岛的样品中检测到。

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