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Effect of delay of blood cultures on positive detection by automated blood culture system

机译：血液培养延迟对自动血液培养系统阳性检测的影响

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The effect of entry delayed blood culture bottles until the start of incubation for mechanical detection of organism were compared using 2 major blood culture systems; BACTEC 9240 system and BacT/ALERT 3D system. Total of 13 bacterial strains; 5 gram-positive cocci, 7 gram-negative bacilli and Candida parapsilosis which were isolated mainly from blood cultures were used as the test strains. BACTEC 92F, 93F and BacT/ALERT FA, FN bottles were used as the blood culture bottles. All the bottles inoculated with the test strains were incubated and evaluated immediately after standing at room temperature for 24, 42, 48, 54 or 72 hours, using the respective automated blood culture systems. All the bottles were subcultured. The effect of entry delay the blood culture bottles for the mechanical detection was observed in many gram-negative organisms in BACTEC 9240 system. The blood cultures were evaluated not to be positive in 4 of the 10 samples on delaying for 24 hours or in any of the samples on delaying for 42 hours in the BACTEC 92F bottles inoculated with Escherichia coli. In Serratia marcescens, the blood cultures were evaluated not to be positive in 5 of the 10 samples on delaying for 24 hours or in any of the samples on delaying for 42 hours in the BACTEC 92F bottles. In Klebsiella pneumoniae, the blood cultures were evaluated not to be positive in 9 of the 10 samples on delaying for 42 hours. In Enterococcus faecalis, Pseudomonas aeruginosa and Proteus mirabilis, the blood cultures were evaluated not to be positive in 5-6 of the 10 samples on delaying for 42 hours. On the other hand, the blood cultures were evaluated to be positive in most of the samples of Acinetobacter calcoaceticus (except 3 of the 10 samples which were evaluated not to be positive) on delaying for 42 hours in BacT/ALERT 3 D system. The samples except part of Streptococcus spp. were detected by subculture in both the bottles. These results indicate that the delayed time of blood culture bottles before inoculation with the test bacterial samples affects the positive detection of blood cultures markedly in the blood culture system. Therefore, the immediate incubation was considered to be necessary.
机译：使用2个主要血液培养系统比较进入延迟血液培养瓶的效果直到有机体机械检测的开始; Bactec 9240系统和Bact / Alert 3D系统。共13个细菌菌株;使用主要从血液培养物分离的5克阳性COCC1，7克阴性杆菌和念珠菌肺纤维化剂作为试管。 Bactec 92F，93F和Bact / Alert Fa，FN瓶用作血液培养瓶。使用各自的自动血液培养系统，在室温下静置24,42,48,54或72小时后立即温育和评价所有接种用测试菌株的瓶子。所有瓶子都被推培养。在Bactec 9240系统中，在许多革兰氏阴性生物中观察到进入延迟血液培养瓶的血液培养瓶。评估血液培养物在10个样品中的4个样品中的4小时中，或者在任何样品中延迟24小时，以在接种大肠杆菌的Bactec 92F瓶中延迟42小时。在Serratia Marcescens中，评估血液培养物在10个样品中的5个时，在延迟24小时或任何样品中，在Bactec 92F瓶中延迟42小时的任何样品中的5个。在Klebsiella肺炎中，评估血液培养物在10个样品中的9个时不呈阳性，延迟42小时。在肠球菌粪便中，假单胞菌铜绿假单胞菌和蛋白质Mirabilis，评价血液培养物在10个样品中的5-6个样品中的液体培养不呈阳性，延迟42小时。另一方面，评估血液培养物在大多数分枝杆菌的样品中阳性（在10个样品中的3种除以阳性的3种除以阳性），在Bact / Alert 3 D系统中延迟42小时。除了链球菌的一部分除了链球菌的样本。通过瓶子中的传代培养来检测。这些结果表明，用测试细菌样品接种前血液培养瓶的延迟时间影响血液培养系统中血液培养的阳性检测。因此，认为需要立即孵化。

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