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Effect of delay of blood cultures on positive detection by automated blood culture system

机译：血培养延迟对自动血培养系统阳性检测的影响

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The effect of entry delayed blood culture bottles until the start of incubation for mechanical detection of organism were compared using 2 major blood culture systems; BACTEC 9240 system and BacT/ALERT 3D system. Total of 13 bacterial strains; 5 gram-positive cocci, 7 gram-negative bacilli and Candida parapsilosis which were isolated mainly from blood cultures were used as the test strains. BACTEC 92F, 93F and BacT/ALERT FA, FN bottles were used as the blood culture bottles. All the bottles inoculated with the test strains were incubated and evaluated immediately after standing at room temperature for 24, 42, 48, 54 or 72 hours, using the respective automated blood culture systems. All the bottles were subcultured. The effect of entry delay the blood culture bottles for the mechanical detection was observed in many gram-negative organisms in BACTEC 9240 system. The blood cultures were evaluated not to be positive in 4 of the 10 samples on delaying for 24 hours or in any of the samples on delaying for 42 hours in the BACTEC 92F bottles inoculated with Escherichia coli. In Serratia marcescens, the blood cultures were evaluated not to be positive in 5 of the 10 samples on delaying for 24 hours or in any of the samples on delaying for 42 hours in the BACTEC 92F bottles. In Klebsiella pneumoniae, the blood cultures were evaluated not to be positive in 9 of the 10 samples on delaying for 42 hours. In Enterococcus faecalis, Pseudomonas aeruginosa and Proteus mirabilis, the blood cultures were evaluated not to be positive in 5-6 of the 10 samples on delaying for 42 hours. On the other hand, the blood cultures were evaluated to be positive in most of the samples of Acinetobacter calcoaceticus (except 3 of the 10 samples which were evaluated not to be positive) on delaying for 42 hours in BacT/ALERT 3 D system. The samples except part of Streptococcus spp. were detected by subculture in both the bottles. These results indicate that the delayed time of blood culture bottles before inoculation with the test bacterial samples affects the positive detection of blood cultures markedly in the blood culture system. Therefore, the immediate incubation was considered to be necessary.
机译：使用两种主要的血液培养系统比较了延迟进入血液培养瓶直至开始进行用于机械检测生物的培养的效果。 BACTEC 9240系统和BacT / ALERT 3D系统。共有13个细菌菌株;主要从血液培养物中分离出的5克阳性球菌，7克阴性杆菌和副念珠菌被用作测试菌株。 BACTEC 92F，93F和BacT / ALERT FA，FN瓶用作血液培养瓶。使用各自的自动血液培养系统，在室温下放置24、42、48、54或72小时后，立即孵育并接种所有接种测试菌株的瓶子。所有的瓶子都进行了亚培养。在BACTEC 9240系统中的许多革兰氏阴性生物中，观察到了进入血培养瓶以进行机械检测的延迟效应。在接种大肠杆菌的BACTEC 92F瓶中，在10个样品中有4个样品在延迟24小时后或在任何一个样品中延迟42小时后，血液培养均未呈阳性。在粘质沙雷氏菌中，在BACTEC 92F瓶中，延迟10小时的10个样品中有5个或延迟42小时的任何样品中的血液培养物均未检测到阳性。在肺炎克雷伯菌中，在延迟42小时后，在10个样本中有9个的血液培养被评估为阴性。在粪便肠球菌，铜绿假单胞菌和奇异变形杆菌中，在延迟42小时后的10个样本中，有5-6个样本的血液培养物被评估为阴性。另一方面，在BacT / ALERT 3D系统中延迟了42小时后，在大多数钙乙酸不动杆菌样品中血液培养被评估为阳性（10个样品中有3个被评估为不是阳性）。除链球菌属的一部分外的样品。通过两个瓶中的亚培养检测到。这些结果表明，在用测试细菌样品接种之前，血液培养瓶的延迟时间会显着影响血液培养系统中血液培养物的阳性检测。因此，认为立即孵育是必要的。

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