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首页> 外文期刊>ACS applied materials & interfaces >Multilayer Dual-Polymer-Coated Upconversion Nanoparticles for Multimodal Imaging and Serum-Enhanced Gene Delivery
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Multilayer Dual-Polymer-Coated Upconversion Nanoparticles for Multimodal Imaging and Serum-Enhanced Gene Delivery

机译：多层双聚合物涂层上转换纳米粒子用于多峰成像和血清增强基因传递。

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摘要

Upconversion nanopartkles (UCNPs) have been widely explored for various bioapplications because of their unique optical properties, easy surface functionalization, and low cytotoxicity. Herein, we synthesize gadolinium (Gd~(3+))-doped UCNPs, which are modified first with poly(ethylene glycol) (PEG) and then with two layers of poly(ethylenimine) (PEI) via covalent conjugation and layer-by-layer assembly, respectively. Compared with UCNP-PEG@1XPEt with only one layer of PEI coating, the final complex, UCNP-PEG@ 2XPEI, with two PEI layers exhibits reduced cytotoxicity and enhanced gene transfection efficiency. It is interesting to find that while free PEI polymer is only effective in gene transfection in a serum-free medium and shows drastically reduced transfection ability if serum is added, UCNP-PEG@2XPE1 is able to transfect cells in both serum-free and -containing media and, surprisingly, offers even higher gene transfection efficiency if serum is added. This is likely due to the formation of protein corona on the nanoparticle surface, which triggers the receptor-mediated endocytosis of our UCNP vectors. Considering the upconversion luminescence and magnetic resonance imaging contrasting ability of UCNPs, our novel nanovector could serve as a "trackable" gene-delivery carrier promising for theranostic applications.
机译：上转换纳米颗粒（UCNPs）由于其独特的光学特性，易于表面功能化和低细胞毒性而被广泛用于各种生物应用。本文中，我们合成了（（Gd〜（3+））掺杂的UCNP，该UCNPs先用聚乙二醇（PEG）修饰，然后通过共价键合和逐层修饰两层聚乙烯亚胺（PEI）修饰层组件。与只有一层PEI涂层的UCNP-PEG @ 1XPEt相比，具有两层PEI层的最终复合物UCNP-PEG @ 2XPEI表现出降低的细胞毒性和增强的基因转染效率。有趣的是，虽然游离的PEI聚合物仅可在无血清培养基中有效转染基因，并且如果添加血清则显示出明显降低的转染能力，但UCNP-PEG @ 2XPE1能够在无血清和-令人惊讶的是，如果添加血清，它可以提供更高的基因转染效率。这可能是由于在纳米粒子表面形成了蛋白质电晕，从而触发了UCNP载体的受体介导的内吞作用。考虑到UCNPs的上转换发光和磁共振成像的对比能力，我们新型的纳米载体可以作为有希望的治疗学应用的“可追踪”基因传递载体。

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