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首页> 外文期刊>ACS applied materials & interfaces >Binding of HIV-1 gp120 Glycoprotein to Silica Nanoparticles Modified with CD4 Glycoprotein and CD4 Peptide Fragments
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Binding of HIV-1 gp120 Glycoprotein to Silica Nanoparticles Modified with CD4 Glycoprotein and CD4 Peptide Fragments

机译：HIV-1 gp120糖蛋白与CD4糖蛋白和CD4肽片段修饰的二氧化硅纳米颗粒的结合。

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摘要

An important step in human immunodeficiency virus infection involves the interaction between the viral envelope glycoprotein gpl20 and the human host cell surface receptor CD4. Herein, we describe a CD4-functionalized mesoporous silica-based system to selectively capture HIV-gpl20 with high binding efficiency. Using a protection-deprotection strategy developed recently by our group, the external surface of the mesoporous particles was selectively functionalized with soluble CD4 ("sCD4") or an 18-peptide fragment mimicking the gpl20 binding region. Confocal microscopy confirmed the CD4 locations and showed that the internal pores can be made accessible after external modification in a controlled manner. An evaluation of the ability of an 18-peptide CD4 fragment versusamide-immobilized sCD4 and sCD4 immobilized through its glycosidic group indicated that while all peptides were selective, the latter method was clearly best, with nearly complete removal of whole gpl20 from solution. This study shows, for the first time, that sCD4 bound to mesoporous silica particles actively recognizes and retains high binding affinity for HIV-gpl20. It is anticipated that, by proper modification of the accessible internal pores, our methodology can be adopted to develop porous platforms for HIV diagnosis, imaging, drug delivery, and vaccine development.
机译：人类免疫缺陷病毒感染的重要步骤涉及病毒包膜糖蛋白gp120和人类宿主细胞表面受体CD4之间的相互作用。在这里，我们描述了一种CD4官能化的介孔二氧化硅基系统，以高结合效率选择性捕获HIV-gp120。使用我们小组最近开发的保护-脱保护策略，用可溶性CD4（“ sCD4”）或模仿gp120结合区的18肽片段选择性地功能化中孔颗粒的外表面。共聚焦显微镜证实了CD4的位置，并表明在以受控方式进行外部修饰后，内部孔可以进入。对通过其糖苷基团固定的18肽CD4片段与酰胺固定的sCD4和sCD4的能力进行的评估表明，尽管所有肽都是选择性的，但后一种方法显然是最好的，几乎可以从溶液中完全去除整个gp120。这项研究首次显示，与中孔二氧化硅颗粒结合的sCD4主动识别并保持对HIV-gp120的高结合亲和力。可以预料的是，通过对可进入内部孔的适当修改，我们的方法可以被采用来开发用于HIV诊断，成像，药物输送和疫苗开发的多孔平台。

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