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首页> 外文期刊>Allergy & clinical immunology international: official organ of the International Association of Allergology and Clinical Immunology >A Model of Transfusion-Related Acute Lung Injury: Roles of IL-8, RANTES, IL-18, and Histamine in the Development of Cultured Human Lung Microvascular Endothelial Cell Injury In Vitro
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A Model of Transfusion-Related Acute Lung Injury: Roles of IL-8, RANTES, IL-18, and Histamine in the Development of Cultured Human Lung Microvascular Endothelial Cell Injury In Vitro

机译：输血相关的急性肺损伤模型：IL-8，RANTES，IL-18和组胺在体外培养的人肺微血管内皮细胞损伤发展中的作用

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Background: Transfusion-related acute lung injury (TRALI) is a rare but serious side effect of transfusion. Upregulated human lung microvascular endothelial (LME) cell membrane permeability is considered to induce edema and respiratory distress. The aim of this study is the identification of soluble mediators that induce the migration and accumulation of polymorphonuclear neutrophils (PMNs) in inflamed sites of LME cells followed by the upregulation of inflammatory reactions by in vitro experiments using cultured human LME cells. Methods/Data base: We measured soluble inflammatory mediators in the culture supernatants of LME cells, monocytes, and the combined culture of LME cells and monocytes by enzyme-linked immunosorbent assay (ELISA). The migration of PMNs was assessed by an in vitro assay using an agarose plate. Results: Significant amounts of interleukin-8 (IL-8) and regulated upon activation normal T cell expressed and secreted (RANTES) were observed in the culture supernatant of the combined culture of LME cells and monocytes. PMN migration was notably enhanced by the culture supernatant of the combined culture of LME cells and monocytes. Moreover, a constitutive release of histamine and IL-18 was seen in LME cells. Conclusions: From this study it was strongly suggested that the association of monocytes with LME cells upregulated the production of inflammatory chemokines from monocytes that cause PMN migration and accumulation at inflammatory sites. Histamine and IL-18 from LME cells may accelerate these series of inflammatory reactions in an autocrine manner.
机译：背景：与输血有关的急性肺损伤（TRALI）是一种罕见但严重的输血副作用。上调的人肺微血管内皮（LME）细胞膜通透性被认为可引起水肿和呼吸窘迫。这项研究的目的是鉴定可溶的介体，该介体诱导多形核中性粒细胞（PMN）在LME细胞发炎部位的迁移和积累，然后通过使用培养的人LME细胞的体外实验上调炎症反应。方法/数据库：我们通过酶联免疫吸附测定（ELISA）测量了LME细胞，单核细胞的培养上清液以及LME细胞与单核细胞的混合培养物中的可溶性炎性介质。通过使用琼脂糖平板的体外测定法评估PMN的迁移。结果：在LME细胞和单核细胞混合培养的培养上清液中观察到大量白细胞介素8（IL-8），并受激活后正常T细胞表达和分泌的调节（RANTES）。 LME细胞和单核细胞联合培养的培养上清液显着增强了PMN迁移。此外，在LME细胞中观察到组胺和IL-18的组成性释放。结论：从这项研究中，强烈提出单核细胞与LME细胞的结合上调了单核细胞引起的炎症趋化因子的产生，从而导致PMN在炎症部位迁移和积累。 LME细胞中的组胺和IL-18可能以自分泌方式加速这些系列的炎症反应。

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