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首页> 外文期刊>American Journal of Physiology >Heterogeneity of calcium stores and elementary release events in canine pulmonary arterial smooth muscle cells.
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Heterogeneity of calcium stores and elementary release events in canine pulmonary arterial smooth muscle cells.

机译：犬肺动脉平滑肌细胞中钙存储的异质性和基本释放事件。

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摘要

To examine the nature of inositol 1,4,5-trisphosphate (IP(3))-sensitive and ryanodine (Ryn)-sensitive Ca(2+) stores in isolated canine pulmonary arterial smooth cells (PASMC), agonist-induced changes in global intracellular Ca(2+) concentration ([Ca(2+)](i)) were measured using fura 2-AM fluorescence. Properties of elementary local Ca(2+) release events were characterized using fluo 3-AM or fluo 4-AM, in combination with confocal laser scanning microscopy. In PASMC, depletion of sarcoplasmic reticulum Ca(2+) stores with Ryn (300 microM) and caffeine (Caf; 10 mM) eliminated subsequent Caf-induced intracellular Ca(2+) transients but had little or no effect on the initial IP(3)-mediated intracellular Ca(2+) transient induced by ANG II (1 microM). Cyclopiazonic acid (CPA; 10 microM) abolished IP(3)-induced intracellular Ca(2+) transients but failed to attenuate the initial Caf-induced intracellular Ca(2+) transient. These results suggest that in canine PASMC, IP(3)-, and Ryn-sensitive Ca(2+) stores are organized into spatially distinct compartments while similar experiments in canine renal arterial smooth muscle cells (RASMC) reveal that these Ca(2+) stores are spatially conjoined. In PASMC, spontaneous local intracellular Ca(2+) transients sensitive to modulation by Caf and Ryn were detected, exhibiting spatial-temporal characteristics similar to those previously described for "Ca(2+) sparks" in cardiac and other types of smooth muscle cells. After depletion of Ryn-sensitive Ca(2+) stores, ANG II (8 nM) induced slow, sustained [Ca(2+)](i) increases originating at sites near the cell surface, which were abolished by depleting IP(3) stores. Discrete quantal-like events expected due to the coordinated opening of IP(3) receptor clusters ("Ca(2+) puffs") were not observed. These data provide new information regarding the functional properties and organization of intracellular Ca(2+) stores and elementary Ca(2+) release events in isolated PASMC.
机译：若要检查肌醇的1,4,5-三磷酸（IP（3））敏感和ryanodine（Ryn）敏感的Ca（2+）存储在孤立的犬肺动脉平滑细胞（PASMC），激动剂诱导的变化使用呋喃2-AM荧光测量全球细胞内Ca（2+）浓度（[Ca（2 +）]（i））。基本的局部Ca（2+）释放事件的属性使用荧光3-AM或荧光4-AM，结合共焦激光扫描显微镜进行了表征。在PASMC中，肌浆网Ca（2+）与Ryn（300 microM）和咖啡因（Caf; 10 mM）的消耗消除了随后的Caf诱导的细胞内Ca（2+）瞬变，但对初始IP（ 3）介导的ANG II（1 microM）引起的细胞内Ca（2+）瞬变。 Cyclopiazonic酸（CPA; 10 microM）废除了IP（3）诱导的细胞内Ca（2+）瞬变，但未能减弱初始Caf诱导的细胞内Ca（2+）瞬变。这些结果表明，在犬PASMC中，IP（3）-和Ryn敏感的Ca（2+）存储组织成空间上不同的区室，而在犬肾动脉平滑肌细胞（RASMC）中的类似实验表明，这些Ca（2+ ）商店在空间上是相连的。在PASMC中，检测到对Caf和Ryn调节敏感的自发局部细胞内Ca（2+）瞬变，表现出类似于先前针对心脏和其他类型的平滑肌细胞中的“ Ca（2+）火花”所述的时空特征。耗尽Ryn敏感的Ca（2+）存储后，ANG II（8 nM）诱导缓慢，持续的[Ca（2 +）]（i）增加起源于细胞表面附近的位置，并通过消耗IP（3）来消除。 ）商店。没有观察到由于IP（3）受体簇（“ Ca（2+）泡芙”）的协调开放所期望的离散量化事件。这些数据提供有关细胞内Ca（2+）存储和基本的Ca（2+）释放事件在孤立的PASMC中的功能特性和组织的新信息。

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