• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Molecular cloning of rabbit organic cation transporter rbOCT2 and functional comparisons with rbOCT1.
【24h】

Molecular cloning of rabbit organic cation transporter rbOCT2 and functional comparisons with rbOCT1.

机译：兔有机阳离子转运蛋白rbOCT2的分子克隆和与rbOCT1的功能比较。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Multiple organic cation transporters (OCTs) are present in rabbit kidney and may play different functional roles. We cloned rabbit OCT2 (rbOCT2) and compared its function with that of rabbit OCT1 (rbOCT1). In transiently transfected COS-7 cells, rbOCT1 and rbOCT2 mediated uptake of [3H]tetraethylammonium (TEA) with K(t) values of 188 and 125 microM, respectively. n-Tetraalkylammonium compounds showed similar affinities for the two homologs, with IC50 values for inhibition of OCT1- and OCT2-mediated [3H]TEA transport, respectively, of 4,538 and 1,395 microM for tetramethylammonium, 88.5 and 3.9 microM for tetrapropylammonium, 13.9 and 5.3 microM for tetrabutylammonium, and 8.8 and 7.6 microM for tetrapentylammonium. However, the transporters had very different affinities for cimetidine (CIM): IC50 of 916 and 5.7 microM for rbOCT1 and rbOCT2, respectively. CIM inhibition of TEA uptake into single S2 segments of rabbit proximal tubule was used to estimate the contributions of OCT1 and OCT2 to basolateral organic cation uptake. The median IC50 for CIM inhibition of TEA uptake was 12.3 microM, suggesting that OCT2 is the major contributor to basolateral organic cation transport in the S2 segment of proximal tubule in rabbit kidney.
机译：兔肾脏中存在多种有机阳离子转运蛋白（OCT），它们可能发挥不同的功能作用。我们克隆了兔OCT2（rbOCT2），并将其功能与兔OCT1（rbOCT1）进行了比较。在瞬时转染的COS-7细胞中，rbOCT1和rbOCT2介导的K [t]分别为188和125 microM的[3H]四乙铵（TEA）摄取。正四烷基铵化合物对两个同源物表现出相似的亲和力，抑制OCT1和OCT2介导的[3H] TEA转运的IC50值分别为：四甲基铵4,538和1,395 microM，四丙基铵88.5和3.9 microM，13.9和5.3。四丁基铵的浓度为microM，四戊基铵的浓度为8.8和7.6 microM。但是，转运蛋白对西咪替丁（CIM）的亲和力却有很大不同：rbOCT1和rbOCT2的IC50分别为916和5.7 microM。 CIM对兔近端小管的单个S2段摄取TEA的抑制作用用于评估OCT1和OCT2对基底外侧有机阳离子摄取的贡献。 CIM抑制TEA摄取的中位IC50为12.3 microM，这表明OCT2是兔肾近端小管S2段基底外侧有机阳离子转运的主要贡献者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号