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首页> 外文期刊>American Journal of Physiology >Expression of intestinal brush-border membrane hydrolases and ferritin after segmental ischemia-reperfusion in rats.
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Expression of intestinal brush-border membrane hydrolases and ferritin after segmental ischemia-reperfusion in rats.

机译：大鼠节段性缺血再灌注后肠刷膜膜水解酶和铁蛋白的表达

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摘要

Jejunal expression of three brush-border membrane (BBM) enzymes, intestinal alkaline phosphatase (IAP), lactose-phlorizin hydrolase (LPH), and sucrase-isomaltase (SI), and a cytosolic protein, ferritin (Ft), was investigated after transient segmental ischemia-reperfusion (I/R). I/R reduced mucosal IAP, LPH, and SI mRNAs to 36%, 11%, and 38% of normal jejunal levels after 3 h of reperfusion and to 22%, 8%, and 51% of normal jejunal levels after 6 h of reperfusion, respectively. Intriguingly, in the internal control jejunum IAP and LPH mRNAs also decreased significantly. LPH and SI mRNA rapidly recovered to levels significantly higher than those of normal jejunum at 12 h, whereas IAP mRNA levels did not recover until 48 h. Enzyme activity paralleled changes in mRNA levels in the ischemic reperfused jejunum. Electrophoretic mobility shift assays showed that I/R significantly increased SI footprinting 1 (SIF1) binding activity. The mobility of one of the DNA-protein complexes was further retarded in the presence of anti-Cdx-2 antibody, suggesting that either Cdx-2 or a related protein was interacting with the SIF1 sequences. Similar to BBM enzymes, cytosolic Ft mRNA and protein were significantly decreased at 3 and 6 h after I/R. By 12 h, Ft mRNA, but not Ft protein, had increased to higher than normal levels. We conclude that a rapid recovery of BBM mRNAs and enzymes occurs in regenerating mucosa after upper villus damage. The increase of SIF1 binding protein activity after I/R may enhance SI, and perhaps LPH, gene transcription. The expression of Ft is regulated at both pretranslational and translational levels.
机译：短暂性过后，研究了三种刷状边界膜（BBM）酶，肠碱性磷酸酶（IAP），乳糖-福禄菌素水解酶（LPH）和蔗糖异麦芽糖酶（SI）的空肠表达以及胞质蛋白铁蛋白（Ft）节段性缺血再灌注（I / R）。 I / R将再灌注3 h后的黏膜IAP，LPH和SI mRNA降低至正常空肠水平的36％，11％和38％，并在6 h后降低至正常空肠水平的22％，8％和51％再灌注。有趣的是，在内部对照中，空肠中的IAP和LPH mRNA也显着下降。 LPH和SI mRNA在12 h时迅速恢复至明显高于正常空肠的水平，而IAP mRNA水平直到48 h才恢复。酶活性与缺血再灌注空肠中mRNA水平的变化平行。电泳迁移率迁移分析表明，I / R显着增加了SI足迹1（SIF1）的结合活性。在抗Cdx-2抗体的存在下，DNA-蛋白质复合物之一的流动性进一步受到抑制，这表明Cdx-2或相关蛋白质正在与SIF1序列相互作用。与BBM酶相似，胞浆Ft mRNA和蛋白在I / R后3和6 h明显降低。到12小时，Ft mRNA升高，但Ft蛋白却没有升高，高于正常水平。我们得出的结论是，上部绒毛损伤后，再生黏膜中发生了BBM mRNA和酶的快速恢复。 I / R后SIF1结合蛋白活性的增加可能会增强SI甚至LPH基因的转录。 Ft的表达受翻译前和翻译水平的调节。

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