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首页> 外文期刊>日本结晶学会志 >Achievement of the Bio-Crystallography Beamline (BL41XU) and Structural Biology Exploited with Undulator X-rays.
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Achievement of the Bio-Crystallography Beamline (BL41XU) and Structural Biology Exploited with Undulator X-rays.

机译：实现生物晶体谱线线（BL41XU）和利用波浪X射线的结构生物学。

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The Bio-Crystallography beamline (BL41XU) dedicated for X-ray crystallography of biological macromolecules is a public beamline opened from October, 1997. The X-ray source is an in-vacuum type standard undulator at SPring-8, and a rotated inclined double-crystal mono-chromator includes a pin-post water cooling silicon crystal on which a tremendous heat load from the undulator deposits. The focal spot in an experimental station has a size of 200 micron and 300 micron in horizontal and vertical directions, respectively. The flux is 5X10~(12) photons/sec for 12.4 keV X-rays at a storage ring current of 100 mA. In order to align optical elements and a diffractometer after every changes of X-ray energy and sample-detector distance, an automated procedure has been developed within the energy range from 6.5 keV to 37.5 keV. The diffractometer specially designed for macromolecular crystallography at BL4lXU is working with a CCD detector and imaging plate detectors. BL41XU has been constructed for two scientific targets: (1) routine structure analyses of biological macromolecules on the MIR-OAS technique and (2) X-ray crystallography on biologically significant supra-complexes and macromolecules crystallized only into a small size less than 50 micron. Almost of beamline commissioning required for the first target have been completed and many crystal structures have already been reported. For the second target of beamline construction, the commissioning will be continued to open the frontier of structural biology.
机译：专用于生物大分子的X射线晶体学的生物晶体谱线线（BL41XU）是从1997年10月开始的公共梁线。X射线源是弹簧8的真空型标准起伏器，旋转倾斜的双倍-Crystal单色电器包括一个销柱水冷硅晶体，其上具有来自波浪沉积物的巨大热量负荷。实验站中的焦点分别具有200微米和300微米的水平和垂直方向。磁通量为5×10〜（12）光子/秒，用于在100 mA的存储环电流下为12.4keV X射线。为了使光学元件和衍射仪对准X射线能量和采样检测器距离的每次变化后，在能量范围内开发了自动化程序，从6.5keV到37.5 kev。专为BL4LXU的大分子晶体设计专门设计的衍射仪与CCD检测器和成像板探测器一起使用。 BL41XU已经为两个科学目标构建：（1）在生物学显着的SUPRA-复合物上的MIR-OAS技术和（2）X射线晶体中的生物大分子常规结构分析，并且大分子仅在小于50微米的小尺寸中结晶。已经完成了第一个目标所需的几乎光束线调试，并且已经报告了许多晶体结构。对于梁线建设的第二个目标，将继续调试，以打开结构生物学的前沿。

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