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首页> 外文期刊>American Journal of Physiology >Reduced AQP1, -2, and -3 levels in kidneys of rats with CRF induced by surgical reduction in renal mass.
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Reduced AQP1, -2, and -3 levels in kidneys of rats with CRF induced by surgical reduction in renal mass.

机译：通过外科手术减少肾脏重量诱发的CRF大鼠肾脏中AQP1，-2和-3水平降低。

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Urinary concentration characteristically decreases in response to a reduction in renal mass in chronic renal failure (CRF). In the present study, we examined whether there are changes in the expression of aquaporins in rats where CRF was induced by 5/6 nephrectomy. Plasma creatinine levels were significantly elevated consistent with significant CRF: 135.7 +/- 15.1 (n = 17, CRF) vs. 33. 9 +/- 1.1 micromol/l (n = 11, sham), P < 0.05. Two weeks after 5/6 nephrectomy, the remnant kidneys were hypertrophied, and total renal mass increased to 65 +/- 3% of sham levels (P < 0.05). Urine production increased markedly from 40 +/- 2 to 111 +/- 3 microliter. min-1. kg-1 in CRF rats (P < 0.05), whereas urine osmolality and solute-free water reabsorption decreased significantly. Quantitative immunoblotting of total kidney membrane fractions revealed a significant decrease in total kidney AQP2 expression in CRF rats to 43 +/- 12% of sham levels (P < 0.05). A similar reduction was observed for AQP1 and AQP3. Furthermore, the increased urine output and decreased urine osmolality persisted in CRF rats despite 7 days treatment with 1-desamino-[8-D-arginine]vasopressin (DDAVP, 0.1 microgram/h sc) compared with untreated sham-operated controls. Also, there was no change in AQP2 expression (which remained at 38 +/- 3% of sham levels, P < 0.05), urine output, or urine osmolality between CRF rats with or without DDAVP treatment. Immunocytochemistry confirmed the decreased AQP2 expression in collecting duct principal cells in CRF rats, with a predominant apical labeling. In conclusion, the results demonstrated that there was a significant vasopressin-resistant downregulation of AQP2 and AQP3 as well as downregulation of AQP1 associated with the polyuria in CRF rats.
机译：慢性肾功能衰竭（CRF）中，尿液浓度会随着肾脏质量的减少而降低。在本研究中，我们检查了5/6肾切除术诱发CRF的大鼠中水通道蛋白表达是否有变化。血浆肌酐水平显着升高，符合显着的CRF：135.7 +/- 15.1（n = 17，CRF）与33. 9 +/- 1.1 micromol / l（n = 11，假），P <0.05。 5/6肾切除术后两周，残余肾脏肥大，总肾脏质量增加至假手术水平的65 +/- 3％（P <0.05）。尿液产量从40 +/- 2微升显着增加到111 +/- 3微升。 min-1。在CRF大鼠中kg-1（P <0.05），而尿渗透压和无溶质水的重吸收明显降低。定量分析总肾膜部分的免疫印迹显示，CRF大鼠总肾脏AQP2表达显着降低至假手术水平的43 +/- 12％（P <0.05）。对于AQP1和AQP3观察到类似的减少。此外，与未经假手术的对照组相比，尽管使用1-desamino- [8-D-精氨酸]加压素（DDAVP，0.1μg/ h sc）治疗7天，CRF大鼠仍持续增加尿量和降低了渗透压。同样，在接受或不接受DDAVP治疗的CRF大鼠之间，AQP2表达（保持在假手术水平的38 +/- 3％，P <0.05），尿量或尿渗透压没有变化。免疫细胞化学证实，CRF大鼠中收集的导管主细胞中的AQP2表达降低，并具有明显的顶端标记。总之，结果表明，CRF大鼠存在明显的抗加压素AQP2和AQP3下调以及与多尿症有关的AQP1下调。

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