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首页> 外文期刊>American Journal of Physiology >Platelets activated by transient coronary occlusion exacerbate ischemia-reperfusion injury in rat hearts.
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Platelets activated by transient coronary occlusion exacerbate ischemia-reperfusion injury in rat hearts.

机译：短暂性冠状动脉闭塞激活的血小板加剧了大鼠心脏的缺血再灌注损伤。

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摘要

Platelets (Plt) accumulate in reperfused myocardium but their effect on myocardial necrosis has not been established. We tested the hypothesis that the effect of Plt depends on their activation status. Pig Plt were obtained before 48 min of coronary occlusion (pre-CO-Plt), 10 min after reperfusion (R-Plt), or after a 60-min sham operation (sham-Plt). Plt were infused into isolated rat hearts (n = 124) and subsequently submitted to 60 min of ischemia and 60 min of reperfusion. P-selectin expression was higher (P = 0.02) in R-Plt than in pre-CO-Plt or sham-Plt. Lactate dehydrogenase (LDH) release during reperfusion was similar in hearts receiving pre-CO-Plt, sham-Plt, or no Plt, but R-Plt increased LDH release by 60% (P = 0.004). Activation of pre-CO-Plt with thrombin increased P-selectin expression and LDH release (P < 0.001), and these results were unaffected by tirofiban. There was a close correlation between P-selectin expression and LDH release (r = 0.84; P < 0.001), and myocardial Plt accumulation (r = 0.85; P < 0.001). We conclude that the deleterious effect of Plt on reperfused myocardium depends on their activation status as represented by P-selectin expression, which is enhanced by ischemia-reperfusion.
机译：血小板（Plt）在再灌注心肌中积累，但尚未确定其对心肌坏死的作用。我们检验了Plt的效果取决于其激活状态的假设。在冠状动脉闭塞前48分钟（pre-CO-Plt），再灌注后10分钟（R-Plt）或假手术60分钟（sham-Plt）后获得猪Plt。将Plt灌注到离体的大鼠心脏中（n = 124），然后进行60分钟的缺血和60分钟的再灌注。 R-Plt中的P-选择素表达高于前CO-Plt或Sham-Plt（P = 0.02）。在接受预CO-Plt，sham-Plt或不接受Plt的心脏中，再灌注过程中的乳酸脱氢酶（LDH）释放相似，但是R-Plt使LDH释放增加了60％（P = 0.004）。凝血酶激活pre-CO-Plt会增加P-选择素的表达和LDH的释放（P <0.001），并且这些结果不受替罗非班的影响。 P-选择蛋白表达与LDH释放（r = 0.84; P <0.001）和心肌Plt积聚（r = 0.85; P <0.001）密切相关。我们得出结论，Plt对再灌注心肌的有害作用取决于其激活状态，如P-选择素表达所代表的那样，缺血再灌注增强了Pltin的表达。

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