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首页> 外文期刊>American Journal of Physiology >Upregulation of the cardiac homeobox gene Nkx2-5 (CSX) in feline right ventricular pressure overload.
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Upregulation of the cardiac homeobox gene Nkx2-5 (CSX) in feline right ventricular pressure overload.

机译：猫右心室压力超负荷时心脏同源盒基因Nkx2-5（CSX）的上调。

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摘要

The recent characterization of the cardiac-specific homeobox gene Nkx2-5 (or CSX) and its detection in normal adult heart tissue raises the possibility of a role in adult hypertrophy. Using pressure overload as a primary stimulus, we used a feline pulmonary artery banding model to produce right ventricular hypertrophy (RVH). Total RNA was hybridized to a full-length murine Nkx2-5 cDNA probe that contained the NK family homeodomain. Nkx2-5 mRNA levels increased 5.1-fold (P < 0.05) and 3.9-fold vs. the corresponding left ventricles at 2 and 7 days of RVH, respectively, during the period of maximal myocardial growth. By 2 wk, when the RVH response had been completed, Nkx2-5 mRNA levels were returning toward baseline. Hybridization with an Nkx2-5 probe not containing the NK homologous homeodomain demonstrated that upregulation was specific for the Nkx2-5 gene. Atrial natriuretic factor and alpha-cardiac actin, both activated in part by Nkx2-5 DNA binding elements, also increased with RVH. These data suggest that a cardiac homeobox gene may play a role in the induction of adult cardiac hypertrophy.
机译：心脏特异性同源盒基因Nkx2-5（或CSX）的最新表征及其在正常成人心脏组织中的检测增加了在成人肥大中起作用的可能性。使用压力超负荷作为主要刺激，我们使用猫肺动脉束带模型产生右心室肥大（RVH）。总RNA与包含NK家族同源结构域的全长鼠Nkx2-5 cDNA探针杂交。在最大心肌生长期间，在RVH的第2天和第7天，与相应的左心室相比，Nkx2-5 mRNA水平分别增加了5.1倍（P <0.05）和3.9倍。到2周时，当RVH反应完成时，Nkx2-5 mRNA水平又回到基线。与不含NK同源同源域的Nkx2-5探针杂交表明，上调对Nkx2-5基因具有特异性。均由Nkx2-5 DNA结合元件部分激活的心钠素和α-心脏肌动蛋白也随RVH升高。这些数据表明心脏同源盒基因可能在诱导成人心脏肥大中起作用。

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