• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI.
【24h】

Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI.

机译：锂诱导的NDI大鼠肾脏集合管细胞细胞组成的变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lithium treatment for 4 wk caused severe polyuria, dramatic downregulation in aquaporin-2 (AQP-2) expression, and marked decrease in AQP-2 immunoreactivity with the appearance of a large number of cells without AQP-2 labeling in the collecting ducts after lithium treatment. Surprisingly, this was not all due to an increase in AQP-2-negative principal cells, because double immunolabeling revealed that the majority of the AQP-2-negative cells displayed [H(+)]ATPase labeling, which identified them as intercalated cells. Moreover, multiple [H(+)]ATPase-labeled cells were adjacent, which was never seen in control rats. Quantitation confirmed a significant decrease in the fraction of collecting duct cells that exhibited detectable AQP-2 labeling compared with control rats: in cortical collecting ducts, 40 +/- 3.4 vs. 62 +/- 1.8% of controls (P < 0.05; n = 4) and in inner medullary collecting ducts, 58 +/- 1.6 vs. 81 +/- 1.3% of controls (P < 0.05; n = 4). In parallel, a significant increase in the fraction of intercalated ([H(+)]ATPase-positive) cells was shown. Urine output, whole kidney AQP-2 expression, cellular organization, and the fractions of principal and intercalated cells in cortex and inner medulla returned to control levels after 4 wk on a lithium-free diet following 4 wk on a lithium-containing diet. In conclusion, lithium treatment not only decreased AQP-2 expression, but dramatically and reversibly reduced the fraction of principal cells and altered the cellular organization in collecting ducts. These effects are likely to be important in lithium-induced nephrogenic diabetes insipidus.
机译：锂治疗4周后导致严重的多尿症，aquaporin-2（AQP-2）表达的急剧下调，以及明显的AQP-2免疫反应性降低，并且锂收集后在收集管中出现大量未标记AQP-2的细胞治疗。出人意料的是，这并非全部归因于AQP-2阴性主细胞的增加，因为双重免疫标记显示大多数AQP-2-阴性细胞显示出[H（+）] ATPase标记，从而将其识别为插入细胞。此外，多个[H（+）] ATPase标记的细胞是相邻的，这在对照组大鼠中从未见过。定量证实与对照大鼠相比，表现出可检测到的AQP-2标记的收集导管细胞分数显着降低：在皮质收集导管中，对照的40 +/- 3.4％vs对照的62 +/- 1.8％（P <0.05; n = 4），而在髓内收集管中，对照组的比例为58 +/- 1.6与对照组的81 +/- 1.3％（P <0.05; n = 4）。平行地，显示出插入的（[H（+）] ATPase-阳性）细胞的分数显着增加。含锂饮食4 wk后，无锂饮食4 wk后，尿量，全肾AQP-2表达，细胞组织以及皮层和内髓质中主要细胞和插层细胞的分数恢复至对照水平。总之，锂处理不仅降低了AQP-2表达，而且显着且可逆地减少了主要细胞的比例并改变了收集管道中的细胞组织。这些作用在锂诱导的尿崩症中可能很重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号